A number of key principles applicable to forensic knot analysis emerged, most of which have been confirmed by other studies. Most notably, tying behavior is consistent and reproducible. Basic knots - like Overhand Knots, Half Hitches and Half Knots - are chiral, and individual knot tiers produce one mirror-image version more so than the other. This tendency can be influenced by a number of factors, such as the number of available working ends, tying position, interference, the creation of slip loops, and wend switching. These principles can guide investigators in the forensic analysis of knotted evidence and point to future avenues of research. (Published Abstract) Figures, table, and references