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NCJ Number: NCJ 236431     Find in a Library
Title: CODIS STR Template Enrichment by Affinity Bead Capture and Its Application in Forensic DNA Analysis
Author(s): Diane J. Rowold, Ph.D. ; Rachel E. Balsam, M.S. ; Michael C. Jablecki, Ph.D.
Date Published: 2011
Page Count: 39
Sponsoring Agency: National Institute of Justice
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2009-DN-BX-K181
Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
Document: PDF 
Type: Report (Study/Research)
Language: English
Country: United States of America
Annotation: This project’s objective was to adapt and explore a technical application as a potential front-end treatment of a forensic sample, particularly a DNA sample that is not currently amenable to conventional methods, so as to improve the probability that such samples could be evaluated with currently validated approaches.
Abstract: This effort was occasioned by the fact that forensic investigations which involve sub-optimal evidentiary DNA samples are often hampered by incomplete and/or ambiguous Combined DNA Index System (CODIS) STR profiles that arise from low-template quantity, degradation (from age and exposure), mixed human source composition, and/or the presence of PCR inhibitors. This project developed a biotinylated oligonucleotide-streptavidin coated magnetic bead capture process that allows for the multiplex capture and PCR amplification of CODIS specific STR loci. Recovered DNA materials from highly degraded DNA samples or significantly fragmented DNA samples were assessed by a comparative analysis of the 13 established CODIS STR loci plus the amelogenin and D2S1338 STR loci. Head-to head comparisons at single to multiple loci were used to determine whether the process impacted fragment-length bias, two-contributor proportion analysis, and eukaryotic versus prokaryotic specificity. Initial results with a minimally optimized system/process indicate that despite the loss of sizeable quantities (from 30-70 percent) of the specific sequence, there were no locus dropouts, minimal allelic dropouts (less than 1 percent), and statistically relevant CODIS STR profiles were generated. In addition, PCR amplification/CE analysis of captured DNA samples did not introduce additional artifacts that might complicate CODIS STR analysis. The process was also successful at quantitatively extracting eukaryotic STR specific alleles from a five-fold excess prokaryotic background. 10 tables, 9 figures, and 29 references
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Victim identification ; Suspect identification ; Investigative techniques ; DNA fingerprinting ; Databases ; NIJ final report
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=258427

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