The method suggests genotyping a specially designed panel of 1000-3000 single nucleotide polymorphisms (SNPs), each with a relatively low (less than 0.1) minor allele frequency (MAF). The rationale of this method is that any individual will carry a specific set of dozens of rare alleles and the complex DNA mixture will carry this particular set only if the one individual is represented in the DNA mixture. The efficiency of the method is evaluated by estimating the probability that a random man will not be excluded (RMNE) from the mixture. When this probability, P(RMNE), is low, one can conclude that the suspect's DNA is present in the DNA mixture. Essentially, a P(RMNE)less than 10(-9) is considered as proof, whereas a P(RMNE) less than 10(-6) is considered strong evidence. For completeness, the study also analyzed the method using the likelihood ratio (LR) approach. The method was analyzed for a variety of conditions and found that generally the method will provide highly significant results even for complex mixtures combining up to 10 individuals. The method performs well even when close relatives (one or two brothers) are present in the complex DNA mixture and when contributors or suspects come from different populations. The authors also found that the method can accurately identify the number of contributors to the mixture, something that in some instances has significant forensic value on its own. (Published Abstract)