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NCJ Number: NCJ 239302   Add to Shopping cart   Find in a Library
Title: High Throughput Mass Spectrometry to Exploit Genetic Differences in Same-Length STR Alleles
  Document URL: PDF 
Author(s): Thomas A. Hall, Ph.D. ; Steven A. Hofstadler, Ph.D.
Date Published: 2011
Page Count: 116
  Annotation: This final report presents the results of research on the design, validation, and utilization of a next generation STR(short tandem repeat) and Y chromosome-STR typing platform using high throughput mass spectrometry.
Abstract: This report presents the results of research that designed, validated, and implemented a new STR and Y-STR typing platform using high throughput mass spectrometry. The research was undertaken because it has been shown that a need exists within the forensics community to derive maximum information from degraded DNA samples which can yield an incomplete set of STR markers. This research had three specific aims: 1) complete the implementation of a new robust STR (short tandem repeat) panel on the Ibis T5000 platform; 2) develop an ESI-MS (electrospray-ionization mass spectrometry) assay for the SWGDAM (Scientific Working Group on DNA Analysis Methods)-recommended Y-STR markers; and 3) characterize polymorphisms in core autosomal STR and Y-STR markers. These goals were accomplished through the analysis of sets of samples to compile nucleotide-polymorphic allele frequencies in the core CODIS STR loci and the standard forensic Y-STR loci, and through the analysis of samples linked by extended family relationships to verify the faithful transmission of polymorphic alleles. This final result of this research was the development of a new technology for analysis of forensic markers that has culminated in an STR assay and associated software prepared for commercialization. Tables, figures, and references
Main Term(s): DNA fingerprinting
Index Term(s): Trace evidence ; Evidence identification and analysis ; Evidence preservation ; Forensics/Forensic Sciences ; Forensic pathology ; Forensic engineering
Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2008-DN-BX-K304
Sale Source: NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
Type: Research (Applied/Empirical)
Country: United States of America
Language: English
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