| NCJ Number: |
242773  |
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| Title: |
Automating the Differential Digestion Method in the Analysis of Sexual Assault Cases Using Selective Degradation |
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| Document: |
PDF |
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| Author(s): |
Helena G. Wong; Jennifer S. Mihalovich; George Sensabaugh |
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| Date Published: |
2013 |
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| Annotation: |
This project developed and tested an automated differential digestion process that uses selective degradation with DNase 1 in order to remove non-sperm DNA from a mixed sample of evidence in a sexual assault case, thus saving time and labor.
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| Abstract: |
The protocol uses 96-well plates and incorporates microscope slide preparations, resulting in high efficiency and high throughput. Although initial evaluations of the DNase process produced lower DNA yield and inferior STR DNA typing quality compared to the conventional differential digestion. This was corrected by optimizing the concentrations of the divalent ions magnesium and calcium, along with the quantity of DNase used. This modification increased both the DNA yield and the quality of the STR DNA typing results. The protocol was tested on samples stored for extended time periods (up to 60 years) and on samples exposed to multiple freeze/thaw cycles or heat and humidity. All samples subjected to the new protocol produced results similar to samples processed by the conventional differential digestion method. The result was the production of sperm fractions with no or minimal epithelial cell carry-over. 7 tables, 29 figures, and 22 references |
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| Main Term(s): |
Criminology |
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| Index Term(s): |
Blood/body fluid analysis; DNA fingerprinting; Forensic sciences; Investigative techniques; NIJ final report; Rape investigations; Sex offense investigations |
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| Grant Number: |
2010-DN-BX-K182 |
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| Sponsoring Agency: |
National Institute of Justice (NIJ)
Washington, DC 20531
NCJRS Photocopy Services
Rockville, MD 20849-6000 |
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| Sale Source: |
NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America |
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| Page Count: |
48 |
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| Format: |
Document; Document (Online) |
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| Type: |
Report (Grant Sponsored); Report (Study/Research) |
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| Language: |
English |
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| Country: |
United States of America |
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To cite this abstract, use the following link:
http://www.ncjrs.gov/App/publications/abstract.aspx?ID=264848 |
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