| NCJ Number: |
254637  |
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| Title: |
Combined Extraction Method for Mitochondrial DNA and Proteins from Hair for Human Identification |
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| Document: |
PDF |
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| Author(s): |
Sydney Corkran; Craig Bartling |
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| Date Published: |
April 2020 |
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| Annotation: |
This is the Final Summary Overview of the methodology and findings of a project with the goal of developing and demonstrating a method for obtaining whole mitochondrial (mt)-genome haplotype and proteomic profiles from a single limited hair sample. |
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| Abstract: |
The project involved the following four tasks: 1) sample collection (head, pubic, and arm hair, as well as saliva); 2) workflow ideation and planning; 3) baseline analysis; and 4) workflow down-selection (testing the work-flows against established metrics for a working set of hair samples). The work-flow ideation and planning (task 3) involved establishing a working “detection limit” for current state-of-the-art methods, i.e., SD (Sodium Dodecanoate) method, Modified SD method, Triple Prep kit, and FASP method. Various hair lengths from three donors were tested, using the four protein extraction methods. The FASP and SD methods resulted in measurable mtDNA yields in all cases. For 1-cm hair lengths, the FASP method outperformed the SD method. The FASP method provided higher quantities of mtDNA than all other combined methods tested. Optimization of various steps of this method improved results. The researchers envision results from this project having an immediate impact on current proteomics research for purposes of human identification. |
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| Main Term(s): |
Forensic sciences |
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| Index Term(s): |
DNA Typing; Hair and fiber analysis; Investigative techniques; NIJ final report; NIJ grant-related documents; NIJ Resources; Suspect identification; Victim identification |
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| Grant Number: |
2017-DN-BX-0159 |
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| Sponsoring Agency: |
National Institute of Justice (NIJ)
Washington, DC 20531
US Dept of Justice NIJ Pub
Washington, DC 20531 |
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| Sale Source: |
US Dept of Justice NIJ Pub
810 Seventh Street, NW
Washington, DC 20531
United States of America |
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| Page Count: |
15 |
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| Format: |
Document; Document (Online) |
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| Type: |
Program/Project Description; Report (Grant Sponsored); Report (Study/Research); Research (Applied/Empirical) |
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| Language: |
English |
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| Country: |
United States of America |
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To cite this abstract, use the following link:
http://www.ncjrs.gov/App/publications/abstract.aspx?ID=277882 |
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