| NCJ Number: |
254756   |
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| Title: |
Development of A Comprehensive Genetic Tool for Identification of Cannabis Sativa Samples for Forensic and Intelligence Purposes |
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| Document: |
PDF |
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| Author(s): |
Rachel M. Houston |
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| Date Published: |
May 2018 |
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| Annotation: |
This study examined the forensic genetic issues associated with the identification and origin determination of Cannabis Sativa. |
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| Abstract: |
A previously reported 15-loci STR multiplex was evaluated. Evaluation results indicated that this STR system is not suitable for forensic identification due to identified issues. A novel 13-loci STR multiplex was developed and optimized for cannabis sativa identification (3500 Genetic analyzer), according to ISFG and SWGDAM recommendations, using primer and multiplex STR design software, along with a gradient PCR approach for optimal annealing temperature determination. As a proof of concept, a custom panel for MPS was designed to interrogate 12 cannabis-specific STR loci by sequence rather than by size. A simple workflow was designed to integrate the custom PCR multiplex into a workflow compatible with the Ion Plus Fragment Library Kit, Ion Chef, and Ion SS system. The study resulted in a preliminary investigation of sequence variation for 12 autosomal STR loci in 16 cannabis samples. Results revealed intra=repeat variation in eight loci where the nominal or size-based allele was identical, but variances were discovered by sequence. Although the panel was not fully optimized and only a small number of samples were evaluated, this study demonstrated that more informative STR typing can be performed on a MS platform. |
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| Main Term(s): |
Forensic sciences |
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| Index Term(s): |
Designer drugs; Drug analysis; Drug detection; National Institute of Justice (NIJ); NIJ final report |
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| Grant Number: |
2015-R2-CX-0030 |
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| Sponsoring Agency: |
National Institute of Justice (NIJ)
Washington, DC 20531
Sam Houston State University
Huntsville, TX 77340
US Dept of Justice NIJ Pub
Washington, DC 20531 |
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| Corporate Author: |
Sam Houston State University
United States of America |
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| Sale Source: |
US Dept of Justice NIJ Pub
810 Seventh Street, NW
Washington, DC 20531
United States of America |
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| Page Count: |
280 |
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| Format: |
Document; Document (Online) |
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| Type: |
Program/Project Description; Report (Grant Sponsored); Report (Study/Research); Research (Applied/Empirical) |
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| Language: |
English |
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| Country: |
United States of America |
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To cite this abstract, use the following link:
http://www.ncjrs.gov/App/publications/abstract.aspx?ID=278990 |
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