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NCJRS Abstract

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1 record(s) found

 

NCJ Number: 254756 Add to Shopping cart Find in a Library
Title: Development of A Comprehensive Genetic Tool for Identification of Cannabis Sativa Samples for Forensic and Intelligence Purposes
Document: PDF
Author(s): Rachel M. Houston
Date Published: May 2018
Annotation: This study examined the forensic genetic issues associated with the identification and origin determination of Cannabis Sativa.
Abstract: A previously reported 15-loci STR multiplex was evaluated. Evaluation results indicated that this STR system is not suitable for forensic identification due to identified issues. A novel 13-loci STR multiplex was developed and optimized for cannabis sativa identification (3500 Genetic analyzer), according to ISFG and SWGDAM recommendations, using primer and multiplex STR design software, along with a gradient PCR approach for optimal annealing temperature determination. As a proof of concept, a custom panel for MPS was designed to interrogate 12 cannabis-specific STR loci by sequence rather than by size. A simple workflow was designed to integrate the custom PCR multiplex into a workflow compatible with the Ion Plus Fragment Library Kit, Ion Chef, and Ion SS system. The study resulted in a preliminary investigation of sequence variation for 12 autosomal STR loci in 16 cannabis samples. Results revealed intra=repeat variation in eight loci where the nominal or size-based allele was identical, but variances were discovered by sequence. Although the panel was not fully optimized and only a small number of samples were evaluated, this study demonstrated that more informative STR typing can be performed on a MS platform.
Main Term(s): Forensic sciences
Index Term(s): Designer drugs; Drug analysis; Drug detection; National Institute of Justice (NIJ); NIJ final report
Grant Number: 2015-R2-CX-0030
Sponsoring Agency: National Institute of Justice (NIJ)
Washington, DC 20531
Sam Houston State University
Huntsville, TX 77340
US Dept of Justice NIJ Pub
Washington, DC 20531
Corporate Author: Sam Houston State University
United States of America
Sale Source: US Dept of Justice NIJ Pub
810 Seventh Street, NW
Washington, DC 20531
United States of America
Page Count: 280
Format: Document; Document (Online)
Type: Program/Project Description; Report (Grant Sponsored); Report (Study/Research); Research (Applied/Empirical)
Language: English
Country: United States of America
To cite this abstract, use the following link:
http://www.ncjrs.gov/App/publications/abstract.aspx?ID=278990

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