THE STUDY REVIEWED CANDIDATE BLOOD CONSTITUENTS TO DETERMINE WHICH HAD THE PROPERTIES TO PERMIT THEIR USE TO MEET PROGRAM OBJECTIVES. IT WAS DETERMINED THAT EIGHT CONSTITUENTS WOULD BE NEEDED TO PROVIDE THE REQUIRED 1-IN-200 DISCRIMINATION CAPABILITY. THE SELECTED CONSTITUENTS WERE SEPARATED ON VARIOUS SUBSTRATES TO ESTABLISH INDIVIDUAL OPTIMUM METHODOLOGIES. THE STUDY EXPLORED THE POTENTIAL FOR SIMULTANEOUS ANALYSIS. THE EIGHT CONSTITUENTS WERE GROUPED ACCORDING TO THE PH AT WHICH THEY WERE NORMALLY SUBJECTED TO ELECTROPHORESIS. THE THREE GROUPS WERE GLO I, PGM, ESD; ADA, EAP, AK; AND GC AND HP. MANY ITERATIONS USING DIFFERING ANALYTICAL CONDITIONS WERE REQUIRED TO DEVELOP THE PROCEDURES YIELDING THE QUALITY OF RESULTS NECESSARY FOR FORENSIC LABORATORY USE. THAT DEVELOPMENT OBJECTIVES HAD BEEN MET WAS SHOWN IN A FEASIBILITY TEST, USING UNKNOWN SAMPLES OF DRIED BLOODSTAINS. FIELD TESTING AT FOUR PARTICIPATING CRIME LABORATORIES DEMONSTRATED THE UTILITY OF THE BLOODSTAIN ANALYSIS SYSTEM IN THE CRIME LABORATORY. THE FEASIBILITY AND DEMONSTRATION TEST REPORTS ALONG WITH A DESCRIPTION OF STUDY METHODS ARE APPENDED. TABLES AND GRAPHS ARE INCLUDED, AND 25 REFERENCES ARE LISTED. (AUTHOR ABSTRACT MODIFIED)