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NCJ Number: 249274 Find in a Library
Title: Label-Free Aptamer-Fluorophore Assembly for Rapid and Specific Detection of Cocaine in Biofluids
Journal: Analytical Chemistry  Volume:86  Issue:22  Dated:November 2014  Pages:11100-11106
Author(s): Daniel Roncanclo; Haixiang Yu; Xiaowen Xu; Shuo Wu; Ran Liu; Joshua Debord; Xinhui Lou; Yi Xiao
Date Published: November 2014
Page Count: 7
Sponsoring Agency: National Institute of Justice (NIJ)
Washington, DC 20531
Grant Number: 2013-DN-BX-K032
Document: HTML
Type: Report (Grant Sponsored); Report (Study/Research); Research (Applied/Empirical)
Format: Article; Document (Online)
Language: English
Country: United States of America
Annotation: This article reports a rapid and specific aptamer-based method for one-step cocaine detection with minimal reagent requirements.
Abstract: The study demonstrated that the sensor developed in the project can detect cocaine within seconds at concentrations as low as 200 nM, which is 50-fold lower than existing assays based on target-induced conformational change. More importantly, the assay used achieved cocaine detection in body fluids, with a limit of detection of 10.4, 18.4, and 36 ìM in undiluted saliva, urine, and serum samples, respectively. The feasibility of aptamer-based detection has been demonstrated with sensors that operate via target-induced conformational change mechanisms, but these have generally exhibited limited target sensitivity. The authors discovered that the cocaine-binding aptamer MNS-4.1 can also bind the fluorescent molecule 2-amino-5,6,7-trimethyl-1,8-naphthyridine (ATMND) and thereby quench its fluorescence. They subsequently introduced sequence changes into MNS-4.1 to engineer a new cocaine-binding aptamer (38-GC) that exhibits higher affinity to both ligands, with reduced background signal and increased signal gain. Using this aptamer, the authors developed a new sensor platform that relies on the cocaine-mediated displacement of ATMND from 38-GC as a result of competitive binding. (Publisher abstract modified)
Main Term(s): Drug detection
Index Term(s): Blood/body fluid analysis; Cocaine; Forensic sciences; Investigative techniques; NIJ grant-related documents; NIJ Resources
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