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NCJ Number: 249318 Find in a Library
Title: Hydrocarbon Profiles Throughout Adult Calliphoridae Aging: A Promising Tool for Forensic Entomology
Journal: Forensic Science International  Volume:245  Dated:December 2014  Pages:65-71
Author(s): Jennifer L. Pechal; Hannah Moore; Falko Drijfhout; M. Eric Benbow
Date Published: December 2014
Page Count: 7
Sponsoring Agency: National Institute of Justice (NIJ)
Washington, DC 20531
Grant Number: 2010-DN-BX-K243
Document: HTML
Type: Report (Grant Sponsored); Report (Study/Research); Research (Applied/Empirical)
Format: Article; Document (Online)
Language: English
Country: United States of America
Annotation: This study identified hydrocarbon profiles associated with the adults of a North American native blow fly species, Cochliomyia macellaria (Fabricius) and a North American invasive species, Chrysomya rufifacies (Macquart).
Abstract: Blow flies (Diptera: Calliphoridae) are typically the first insects to arrive at human remains and carrion. Predictable succession patterns and known larval development of necrophagous insects on vertebrate remains can assist a forensic entomologist with estimates of a minimum post-mortem interval (PMImin) range. However, adult blow flies are infrequently used to estimate the PMImin, but rather are used for a confirmation of larval species identification. Cuticular hydrocarbons have demonstrated potential for estimating adult blow fly age, as hydrocarbons are present throughout blow fly development, from egg to adult, and are stable structures. The current study This work provides empirical data that serve as a foundation for future research into improving PMImin estimates made by forensic practitioners and potentially increase the use of adult insects during death investigations. Flies were reared at a constant temperature (25 °C), a photoperiod of 14:10 (L:D) (h), and were provided water, sugar and powdered milk ad libitum. Ten adult females from each species were collected at day 1, 5, 10, 20, and 30 post-emergence. Hydrocarbon compounds were extracted and then identified using gas chromatography–mass spectrometry (GC–MS) analysis. A total of 37 and 35 compounds were detected from C. macellaria and Ch. rufifacies, respectively. There were 24 and 23 n-alkene and methyl-branched alkane hydrocarbons from C. macellaria and Ch. rufifacies, respectively (10 compounds were shared between species), used for statistical analysis. Non-metric multidimensional scaling analysis and permutational multivariate analysis of variance were used to analyze the hydrocarbon profiles with significant differences (P < 0.001) detected among post-emergence age cohorts for each species, and unique hydrocarbon profiles detected as each adult blow fly species aged. (Publisher abstract modified)
Main Term(s): Forensic sciences
Index Term(s): Death investigations; Investigative techniques; NIJ grant-related documents; NIJ Resources; Time of death determination
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