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NCJ Number: 249333 Find in a Library
Title: High Sensitivity Multiplex Short Tandem Repeat Loci Analyses With Massively Parallel Sequencing
Journal: Forensic Science International - Genetics  Volume:16  Dated:May 2015  Pages:38-47
Author(s): Xiangpei Zeng; Jonathan L. King; Monika Stoliarova; David H. Warshauer; Bobby L. LaRue; Antti Sajantila; Jaynish Patel; Douglas R. Storts; Bruce Bruce
Date Published: March 2015
Page Count: 10
Sponsoring Agency: National Institute of Justice (NIJ)
Washington, DC 20531
Grant Number: 2012-DN-BXK033
Document: HTML
Type: Report (Grant Sponsored); Report (Study/Research); Research (Applied/Empirical); Test/Measurement
Format: Article; Document (Online)
Language: English
Country: United States of America
Annotation: In this study, a prototype multiplex STR System (Promega) was amplified and prepared using the TruSeq DNA LT Sample Preparation Kit (Illumina) in 24 samples.
Abstract: STR typing in forensic genetics has been performed traditionally using capillary electrophoresis (CE); however, CE-based method has some limitations: a small number of STR loci can be used; stutter products, dye artifacts and low level alleles. Massively parallel sequencing (MPS) has been considered a viable technology in recent years allowing high-throughput coverage at a relatively affordable price. Some of the CE-based limitations may be overcome with the application of MPS. The results of the current study showed that the MinElute PCR Purification Kit (Qiagen) was a better size selection method compared with recommended diluted bead mixtures. The library input sensitivity study determined that a wide range of amplicon product (6–200 ng) could be used for library preparation without apparent differences in the STR profile. PCR sensitivity study indicated that 62 pg may be minimum input amount for generating complete profiles. Reliability study results on 24 different individuals showed that high depth of coverage (DoC) and balanced heterozygote allele coverage ratios (ACRs) could be obtained with 250 pg of input DNA, and 62 pg could generate complete or nearly complete profiles. These studies indicate that this STR multiplex system and the Illumina MiSeq can generate reliable STR profiles at a sensitivity level that competes with current widely used CE-based method. (Publisher abstract modified)
Main Term(s): Forensic sciences
Index Term(s): DNA (Deoxyribonucleic Acid); DNA Typing; Investigative techniques; NIJ grant-related documents; NIJ Resources; Suspect identification; Victim identification
To cite this abstract, use the following link:
http://www.ncjrs.gov/App/publications/abstract.aspx?ID=271477

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