skip navigation

LIBRARY

Abstract Database

Register for Latest Research

Stay Informed
Register with NCJRS to receive NCJRS's biweekly e-newsletter JUSTINFO and additional periodic emails from NCJRS and the NCJRS federal sponsors that highlight the latest research published or sponsored by the Office of Justice Programs.

NCJRS Abstract

To download this abstract, check the box next to the NCJ number then click the "Back To Search Results" link. Then, click the "Download" button on the Search Results page. Also see the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.

 

NCJ Number: 249352 Find in a Library
Title: Massively Parallel Sequencing of Forensically Relevant Single Nucleotide Polymorphisms Using TruSeq (TM) forensic amplicon
Journal: International Journal of Legal Medicine  Volume:129  Issue:1  Dated:January 2015  Pages:31-36
Author(s): David H. Warshauer; Carey P. Davis; Cydne Holt; Yonmee Han; Paulina Walichiewicz; Tom Richardson; Kathryn Stephens; Anne Jager; Jonathan L. King; Bruce Budowle
Date Published: January 2015
Page Count: 6
Sponsoring Agency: National Institute of Justice (NIJ)
Washington, DC 20531
Grant Number: 2012-DN-BX-K033
Document: HTML
Type: Program/Project Description; Report (Grant Sponsored); Report (Study/Research); Report (Technical); Research (Applied/Empirical)
Format: Article; Document (Online)
Language: English
Country: United States of America
Annotation: This study used the TruSeq™ Forensic Amplicon library preparation protocol to detect 160 single nucleotide polymorphisms (SNPs), including human identification SNPs (iSNPs), ancestry, and phenotypic SNPs (apSNPs) in 12 reference samples.
Abstract: The TruSeq™ Forensic Amplicon library preparation protocol, originally designed to attach sequencing adapters to chromatin-bound DNA for chromatin immunoprecipitation sequencing (TruSeq™ ChIP-Seq), was used here to attach adapters directly to amplicons containing markers of forensic interest. Results were com- pared with those generated by a second laboratory using the same technique, as well as to those generated by whole genome sequencing (WGS). The genotype calls made using the TruSeq™ Forensic Amplicon library preparation protocol were highly concordant. The protocol described herein represents an effective and relatively sensitive means of preparing amplified nuclear DNA for massively parallel sequencing (MPS). (Publisher abstract modified)
Main Term(s): Forensics/Forensic Sciences
Index Term(s): DNA (Deoxyribonucleic Acid); DNA Typing; Investigative techniques; NIJ grant-related documents; NIJ Resources; Suspect identification; Victim identification
To cite this abstract, use the following link:
http://www.ncjrs.gov/App/publications/abstract.aspx?ID=271496

*A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's website is provided. Tell us how you use the NCJRS Library and Abstracts Database - send us your feedback.