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NCJ Number: 249352 Find in a Library
Title: Massively Parallel Sequencing of Forensically Relevant Single Nucleotide Polymorphisms Using TruSeq (TM) forensic amplicon
Journal: International Journal of Legal Medicine  Volume:129  Issue:1  Dated:January 2015  Pages:31-36
Author(s): David H. Warshauer; Carey P. Davis; Cydne Holt; Yonmee Han; Paulina Walichiewicz; Tom Richardson; Kathryn Stephens; Anne Jager; Jonathan L. King; Bruce Budowle
Date Published: January 2015
Page Count: 6
Sponsoring Agency: National Institute of Justice (NIJ)
Washington, DC 20531
Grant Number: 2012-DN-BX-K033
Document: HTML
Type: Program/Project Description; Report (Grant Sponsored); Report (Study/Research); Report (Technical); Research (Applied/Empirical)
Format: Article; Document (Online)
Language: English
Country: United States of America
Annotation: This study used the TruSeq™ Forensic Amplicon library preparation protocol to detect 160 single nucleotide polymorphisms (SNPs), including human identification SNPs (iSNPs), ancestry, and phenotypic SNPs (apSNPs) in 12 reference samples.
Abstract: The TruSeq™ Forensic Amplicon library preparation protocol, originally designed to attach sequencing adapters to chromatin-bound DNA for chromatin immunoprecipitation sequencing (TruSeq™ ChIP-Seq), was used here to attach adapters directly to amplicons containing markers of forensic interest. Results were com- pared with those generated by a second laboratory using the same technique, as well as to those generated by whole genome sequencing (WGS). The genotype calls made using the TruSeq™ Forensic Amplicon library preparation protocol were highly concordant. The protocol described herein represents an effective and relatively sensitive means of preparing amplified nuclear DNA for massively parallel sequencing (MPS). (Publisher abstract modified)
Main Term(s): Forensic sciences
Index Term(s): DNA (Deoxyribonucleic Acid); DNA Typing; Investigative techniques; NIJ grant-related documents; NIJ Resources; Suspect identification; Victim identification
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