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NCJ Number: 250564 Find in a Library
Title: Aptamer-Based, Exonuclease-Amplified, Paper Device for Point of Collection Screening of Cocaine and Methamphetamine in Oral Fluid
Author(s): Yi Xiao
Corporate Author: Florida International University
United States of America
Date Published: August 2017
Page Count: 17
Sponsoring Agency: Florida International University
Miami, FL 33199
National Institute of Justice (NIJ)
Washington, DC 20531
US Dept of Justice NIJ Pub
Washington, DC 20531
Grant Number: 2013-DN-BX-K032
Sale Source: US Dept of Justice NIJ Pub
810 Seventh Street, NW
Washington, DC 20531
United States of America
Document: PDF
Type: Program/Project Description; Report (Grant Sponsored); Report (Study/Research); Research (Applied/Empirical)
Format: Document; Document (Online)
Language: English
Country: United States of America
Annotation: In order to simultaneously detect trace amounts of drug molecules in oral fluid with high specificity, this project proposed a colorimetric detection platform based on a low-cost, portable, paper-based micro fluidic device for simultaneously detecting trace amounts of drug molecules (cocaine and methamphetamine in this project).
Abstract: The project’s plan was to generate new aptamers that specifically bind to the targeted drug so as to achieve better specificity than immunoassays. Exonuclease III (Exo III) was used to digest the target/aptamer complex and recycle the target, resulting in high sensitivity and a reduction in false negatives. Detection was to be achieved with a color change that results from gold Nan particle (AuNP) aggregation. By transferring this assay into a portable, postage-stamp-sized, paper-based device, the project anticipated achieving instrument-free, simultaneous detection of cocaine and methamphetamine at low ng/mL concentrations in mL volumes of oral fluid with a visible red-to-blue color change. The project successfully developed a specific, EATR-amplified and AuNP-reported screening platform for cocaine, using a newly engineered cocaine-binding CBSA. Colorimetric detection of 1 uM cocaine was achieved within 15 minutes. The project also modified different colorimetric assays for use in a six-channel, paper-based, microfluidic device. This paper microfluidic device can detect multiple drugs of abuse within 5 minutes. The paper chips are stable for up to 3 months. In parallel, the project also developed a rapid assay based on split aptamers for the detection of cocaine and is in the process of adapting this assay for use in a paper-based microfluidic device. 7 figures, 16 references, and a listing of publications and presentations on the project
Main Term(s): Drug analysis
Index Term(s): Cocaine; Drug testing; Methamphetamines; National Institute of Justice (NIJ); NIJ final report; Saliva sample analysis
To cite this abstract, use the following link:
http://www.ncjrs.gov/App/publications/abstract.aspx?ID=272730

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