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NCJRS Abstract

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NCJ Number: 119555 Find in a Library
Title: Application of Immunoblotting to the Phenotyping of Group-specific Component
Journal: Journal of the Forensic Science Society  Volume:29  Issue:3  Dated:(May/June 1989)  Pages:197-205
Author(s): A S Thomas; A J Ansford
Date Published: 1989
Page Count: 8
Type: Report (Technical)
Format: Article
Language: English
Country: United Kingdom
Annotation: A sensitive immunoblotting method for the routine detection of group-specific component (GC) from fresh serum, and from control and casework bloodstains has been developed.
Abstract: GC phenotypes were separated in a thin layer polyacrylamide gel by isoelectric focusing, transferred to nitrocellulose by a rapid capillary blotting procedure, and detected using a double antibody enzyme immunoassay. This method is capable of phenotyping 8 ng of GC extracted from bloodstains, a fourfold increase in sensitivity when compared to immunofixation and silverstaining. A total of 2424 casework bloodstains have been analysed and GC phenotypes identified in 78 percent of samples. The method is suitable for use in routine laboratories and is more sensitive than other methods for GC phenotyping of casework bloodstains. 2 tables, 5 figures and 15 references. (Publisher abstract)
Main Term(s): Blood stains
Index Term(s): Blood/body fluid analysis
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