skip navigation


Register for Latest Research

Stay Informed
Register with NCJRS to receive NCJRS's biweekly e-newsletter JUSTINFO and additional periodic emails from NCJRS and the NCJRS federal sponsors that highlight the latest research published or sponsored by the Office of Justice Programs.

NCJRS Abstract

The document referenced below is part of the NCJRS Virtual Library collection. To conduct further searches of the collection, visit the Virtual Library. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.


NCJ Number: 121235 Find in a Library
Title: DNA Typing From Single Hairs
Journal: Nature  Volume:332  Dated:(April 7, 1988)  Pages:543-546
Author(s): R Higuchi; C H von Beroldingen; G F Sensabaugh; H A Erlich
Date Published: 1988
Page Count: 4
Type: Report (Technical)
Format: Article
Language: English
Country: United States of America
Annotation: The polymerase chain reaction has been successfully used to detect polymorphic deoxyribonucleic acid (DNA) sequences from single human hairs.
Abstract: This technique permits specific short regions of a gene to be greatly amplified in vitro from as little as a single molecule of DNA. Through this technique, genetically variable mitochondrial and nuclear DNA sequences have been detected from the root region of shed hair as well as from freshly-plucked, single hairs. These sequences have also been detected in a sample from a single hair shaft. Three methods of DNA typing have been used: the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing. These approaches differ from the most common method of DNA analysis, restriction fragment length polymorphism, which requires much larger samples than can be obtained from most forensic samples, such as single hairs and bloodstains or from anthropological, genetic, or zoological samples collected in the field. However, all the DNA identification techniques have produced significant advances in gene and disease mapping and in the forensic identification of individuals. Figures and 35 references. (Author abstract modified)
Main Term(s): DNA fingerprinting
Index Term(s): Forensic sciences; Hair and fiber analysis; Suspect identification
To cite this abstract, use the following link:

*A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's website is provided. Tell us how you use the NCJRS Library and Abstracts Database - send us your feedback.