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NCJRS Abstract

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NCJ Number: 123041 Find in a Library
Title: Phenotyping of x-2-HS Glycoprotein in Bloodstains by Isoelectric Focusing and Immunoblotting
Journal: Journal of Forensic Science Society  Volume:29  Issue:5  Dated:(September-October 1989)  Pages:325-330
Author(s): A Thomas
Date Published: 1989
Page Count: 5
Type: Survey
Format: Article
Language: English
Country: United States of America
Annotation: A sensitive immunoblotting procedure has been applied to the detection of a x-2-HS-glycoprotein (A2HS) phenotypes from control and casework bloodstains.
Abstract: A2HS phenotypes were separated by thin layer polyacrylamide gel isoelectric focusing (PAGIEF) in gels containing Pharmalyte pH 4.2-4.9. After transfer to nitrocellulose by a rapid capillary blot, the A2HS phenotypes were developed using a double antibody enzymeimmunoassay. The evaluation of A2HS phenotyping of casework material was undertaken in parallel with phosphoglucomutase (PGM) phenotyping by PAGIEF. A total of 598 water extracts from casework bloodstains have been tested. Positive results were obtained in 84 percent and 75 percent of samples for PGM and A2HS respectively. The A2HS gene frequencies A2HS*1 = 0.6420, A2HS*2 = 0.3530, and A2HS*3 = 0.0050 were determined from a survey of 1000 people in Brisbane. 3 figures, 1 table, 11 references. (Publisher abstract)
Main Term(s): Blood stains; Blood/body fluid analysis
Index Term(s): Crime laboratories; Evidence identification; Forensic medicine
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