skip navigation


Register for Latest Research

Stay Informed
Register with NCJRS to receive NCJRS's biweekly e-newsletter JUSTINFO and additional periodic emails from NCJRS and the NCJRS federal sponsors that highlight the latest research published or sponsored by the Office of Justice Programs.

NCJRS Abstract

The document referenced below is part of the NCJRS Virtual Library collection. To conduct further searches of the collection, visit the Virtual Library. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.


NCJ Number: 134467 Find in a Library
Title: Improved Method for Subtyping Pi in Old Bloodstains
Journal: Journal of Forensic Sciences  Volume:37  Issue:1  Dated:(January 1992)  Pages:323-327
Author(s): I Lopez-Abadia; E Arroyo; A Gremo; J M Ruiz de la Cuesta
Date Published: 1992
Page Count: 5
Type: Report (Technical)
Format: Article
Language: English
Country: United States of America
Annotation: Immunofixation procedures used to detect alpha-1 antitrypsin protease inhibitor (Pi) in bloodstains have proven to be reliable for subtyping bloodstains up to 12 weeks old and useful for routine case work.
Abstract: Polyacrylamide gel isoelectric focusing was carried out under the following conditions. The gel was 200 by 115 by 0.5 mm in size and contained 6.2 percent in total concentration of monomer and 3.2 percent in the total monomer due to a cross-linking agent. Blood was obtained from 100 normal donors with known Pi types. Stains were made by soaking pieces of clean cotton cloth in whole fresh blood and storing them at room temperature until used. A neuraminidase solution was used for extraction. Pi phenotypes from the 100 bloodstains were determined by isoelectric focusing techniques every week for a 4.5-month period. Neuraminidase elution of bloodstains, together with isoelectric focusing, immunofixation, and silver staining techniques, made Pi subtyping in old bloodstains possible. No extra bands appeared when the storage time was no longer than 3 months. 8 references and 5 figures (Author abstract modified)
Main Term(s): Blood/body fluid analysis
Index Term(s): Criminal investigation; Forensic sciences; Tissue analysis
To cite this abstract, use the following link:

*A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's website is provided. Tell us how you use the NCJRS Library and Abstracts Database - send us your feedback.