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NCJRS Abstract

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NCJ Number: 136029 Find in a Library
Title: Effect of Storage Conditions on Restriction Fragment Length Polymorphism (RFLP) Analysis of Deoxyribonucleic Acid (DNA) Bound to Positively Charged Nylon Membranes
Journal: Journal of Forensic Sciences  Volume:37  Issue:2  Dated:(March 1992)  Pages:597-603
Author(s): A M Giusti; B Budowle
Date Published: 1992
Page Count: 7
Type: Test/Measurement
Format: Article
Language: English
Country: United States of America
Annotation: This study describes the storage conditions for DNA bound to positively charged nylon membranes that impact positively and negatively on the ability to obtain restriction-fragment-length-polymorphism (RFLP) results.
Abstract: Human DNA from whole blood samples was isolated and analyzed according to procedures described by Budowle and Baechtel. Immediately after the DNA was fixed on the nylon membranes by heat baking at 80 degrees C for 30 minutes, the membranes were analyzed by hybridization with VNTR-specific probes or stored under various conditions. The membranes were stored under -20 degrees C, 4 degrees C, and ambient temperature and humidity. After varying times, the membranes were rehybridized to examine the effect of the various storage conditions. The concern was that storage conditions for the membranes permit RFLP profiles for the immobilized DNA over an extended period of time. Based upon study results, two brands of positively charged membranes examined can be stored at -20 degrees C or at 4 degrees C without any significant loss of detection sensitivity. Storage should be in sealed plastic bags or by thorough wrapping in plastic wrap at -20 degrees C to reduce the possibility of bacterial or fungal growth which might affect the integrity of the membrane or human DNA. 2 figures and 8 references
Main Term(s): DNA fingerprinting
Index Term(s): Evidence preservation
Note: This is Publication 91-09 of the Laboratory Division of the FBI.
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