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NCJRS Abstract

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NCJ Number: 136861 Find in a Library
Title: Simultaneous Diagnosis of Coagulation Factor XIIIA (F13A) and Plasminogen (PLG) Phenotypes by Polyacrylamide Gel Isoelectric Focusing
Journal: Journal of Forensic Sciences  Volume:37  Issue:3  Dated:(May 1992)  Pages:695-699
Author(s): J L B Caeiro; C Llano; E Parra
Date Published: 1992
Page Count: 5
Type: Report (Technical)
Format: Article
Language: English
Country: United States of America
Annotation: The increasing number of electrophoretic genetic markers has emphasized the need for simultaneous determination techniques in human genetic studies and forensic science analysis, and this paper reports a method for simultaneous determination of coagulation factor XIIIA (F13A) and plasminogen (PLG) phenotypes by isoelectric focusing.
Abstract: Isoelectric focusing, as a method for molecular separation, is particularly useful since the proteins remain immobilized (focused) in their respective isoelectric points. This aspect is relevant to the simultaneous analysis of genetic markers provided that the corresponding isoelectric points are defined at intervals sufficiently separate and therefore easily distinguished, without causing problems of pattern interpretation. To evaluate isoelectric focusing in combination with immunofixation as a strategy for specific detection and characterization of both polymorphic systems, PLG and F13A, blood samples were obtained from healthy individuals and treated with ethylenediaminetetraacetate-disodium. Results showed that PLG appeared as a multiple-banded pattern. Neuraminidase pretreatment of plasma samples facilitated the distinction of PLG phenotypes. It is concluded that isoelectric focusing is the technique of choice when dealing with simultaneous determination of both PLG and F13A phenotypes. 14 references and 2 figures (Author abstract modified)
Main Term(s): Blood/body fluid analysis
Index Term(s): Criminal investigation; Forensic medicine
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