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NCJRS Abstract

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NCJ Number: 140068 Find in a Library
Title: ABO Genotyping by Polymerase Chain Reaction
Journal: Journal of Forensic Sciences  Volume:37  Issue:5  Dated:(September 1992)  Pages:1269-1275
Author(s): J C-I Lee; J-G Chang
Date Published: 1992
Page Count: 7
Type: Report (Technical)
Format: Article
Language: English
Country: United States of America
Annotation: This study demonstrated the applicability of molecular genetic techniques to ABO genotype analysis directly from the primary structure of DNA.
Abstract: DNA was isolated from 20 ABO-known peripheral blood samples, blood stains, saliva stains, hair, bone, and vaginal swab. Positions of nucleotide 258 and 700 of cDNA from A transferase were used to distinguish A, B, and O alleles by restriction enzyme digestion. ABO genotypes were easily determined by examining DNA fragment digestion patterns. Results obtained using this method on 20 ABO-known peripheral blood samples showed that the technique provided accurate ABO genotype. Positions of nucleotide 258 and 700 were enough to determine ABO polymorphism. Several advantages of using the method are noted: ABO genotype can be determined; the genomic DNA typing method provides definite results; in rape cases, the approach can definitely determine the rapist's blood group; and the method is simple, fast, economic, sensitive, and reliable. 22 references, 2 tables, and 3 figures
Main Term(s): Blood/body fluid analysis
Index Term(s): Blood stains; Criminal investigation; DNA fingerprinting; Evidence identification; Forensic medicine; Forensic pathology; Polymer analysis; Rape investigations
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