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NCJRS Abstract

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NCJ Number: 140915 Find in a Library
Title: Extraction, Evaluation, and Amplification of DNA From Decalcified and Undecalcified United States Civil War Bone
Journal: Journal of Forensic Sciences  Volume:38  Issue:1  Dated:(January 1993)  Pages:60-68
Author(s): D L Fisher; M M Holland; L Mitchell; P S Sledzik; A W Wilcox; M Wadhams; V W Weedn
Date Published: 1993
Page Count: 9
Type: Report (Technical)
Format: Article
Language: English
Country: United States of America
Annotation: Deoxyribonucleic acid (DNA) was extracted from documented skeletal specimens of U.S. Civil War soldiers to determine the need for decalcification prior to extraction.
Abstract: The polymerase chain reaction (PCR) was performed to determine if calcification state affected the ability to amplify extracts and to see how successful amplification would be with aged specimens. One set of samples was decalcified, while another set was left undecalcified. Both sets were extracted using an organic procedure. Results demonstrated that decalcification was not a necessary step in the extraction process and that DNA yield was generally two times greater when decalcification was omitted. Further, calcification state had no effect on the ability to perform the PCR. Although extracted DNA was very degraded, a 410 base pair segment of the mitochondrial DNA control region was amplified. The authors conclude thaat DNA can be extracted and amplified from 125-year-old bone without decalcification and that this process may assist in identifying modern and historic forensic specimens. 26 references, 3 tables, and 1 figure
Main Term(s): Bone analysis
Index Term(s): Criminal investigation; Evidence identification; Forensic anthropology; Victim identification
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