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NCJRS Abstract

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NCJ Number: 140941 Find in a Library
Title: Presence of a PCR-Inhibitor in Hairs
Journal: Japanese Journal of Legal Medicine  Volume:46  Issue:5  Dated:(October 1992)  Pages:313-316
Author(s): T Yoshii; K Tamura; I Ishiyama
Date Published: 1992
Page Count: 4
Type: Research (Applied/Empirical)
Format: Article
Language: English; Japanese
Country: Japan
Annotation: A polymerese chain reaction (PCR) system was used to amplify the noncoding 333-bp region of mitochondrial DNA (mt333DNA) contained in extracts from single human hairs.
Abstract: The findings showed that, when using naturally black hairs, mt333DNA was always amplified from a 5-centimeter length of hair sampled within a region 11 centimeters from the hair root but was not always amplified from a region adjacent to the hair root and was hardly ever amplified from regions further away from the hair root. When using naturally white hairs, mt333DNA was amplified from all specimens up to a length of 31 centimeters. When naturally black hairs were stained with an oxidation-type hair-staining formula, mt333DNA was never amplified even from the hair root region, although staining white hairs did not affect the amplification of mt333DNA. Dark brown DNA preparations, used in cases showing no response on PCR, completely inhibited the amplification of mt333DNA even after addition of purified DNA. The researchers found that hydrogen peroxide induced formation of water- soluble melanins from insoluble melanins, suggesting that the formation of water-soluble melanins, which occur in naturally black hairs, may be caused by oxidation with air. 3 tables and 6 references
Main Term(s): Hair and fiber analysis
Index Term(s): Polymer analysis
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