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NCJRS Abstract

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NCJ Number: 163582 Find in a Library
Title: Removal of PCR Inhibitors From Forensic Samples and Enhanced Sensitivity for DIS80 Using Fluorescent Scanning
Journal: Canadian Society of Forensic Science Journal  Volume:29  Issue:1  Dated:(March 1996)  Pages:29-32
Author(s): J Makuch; E Brucker; R Schmid; P Mynahan; T Owen
Date Published: 1996
Page Count: 4
Type: Report (Technical)
Format: Article
Language: English
Country: Canada
Annotation: This study found that polymerase chain reaction results may not always be obtained for forensic samples even though sufficient DNA appears to be present by slot blot, and the researchers determined that Taq polymerase inhibitors present in samples may have produced the results.
Abstract: DNA was extracted using standard organic methods and ethanol precipitation and then quantified by stained yield gels and slot blot techniques. Typical samples in the experiments were obtained from two sets of decalcified human bones recovered from fresh water after 3 to 5 years, blood in fabric, blood on metal, and the flap of a sealed envelope. Using a combination of sample dilution, Thiopropyl Sepharose beads, BSA, and additional Taq polymerase made it possible to overcome inhibition of the polymerase. Although this treatment was used with some success in the laboratory, the researchers caution that it does not work with every sample. 2 references and 1 figure
Main Term(s): Science and Technology
Index Term(s): Blood/body fluid analysis; Bone analysis; Criminal investigation; Criminalistics; DNA fingerprinting; Evidence identification; Forensic medicine; Forensic sciences; Hair and fiber analysis
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