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NCJRS Abstract

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NCJ Number: 164951 Find in a Library
Title: HLA DQA1 and Polymarker Validations for Forensic Casework: Standard Specimens, Reproducibility, and Mixed Specimens
Journal: Journal of Forensic Sciences  Volume:41  Issue:6  Dated:(November 1996)  Pages:1022-1026
Author(s): A M Gross; R A Guerrieri
Date Published: 1996
Page Count: 5
Type: Report (Technical)
Format: Article
Language: English
Country: United States of America
Annotation: This study describes the testing performed by the Minnesota Forensic Science Laboratory (MFSL) to validate the Amplitype DQA1 and Amplitype Polymarker PCR (polymerase chain reaction) Amplification and Typing Kits before implementation for casework.
Abstract: The research was based on the analysis of mock forensic case samples that included various biological samples from individuals at the MFSL. To address the validation of standard specimens, DNA was isolated from semen, vaginal secretions, saliva, urine, and blood samples. Typing results from all tissues from a particular individual yielded the same typing results using both DQA1 and Polymarker systems. Reproducibility between laboratories was evaluated by having duplicate samples analyzed by a second laboratory. All typing results from both laboratories for approximately 30 single source samples were in agreement. Mixed specimens were evaluated by examining results obtained from semen/vaginal, semen/saliva, semen/blood, semen/urine, and semen/vaginal/blood mixtures. All typing results from both laboratories for these mixtures were in agreement. It was determined that, by incorporating a wash step of the sperm cell pellet, a complete separation of the nonsperm cell fraction was more likely to be attained. 20 references and 3 tables
Main Term(s): Science and Technology
Index Term(s): Blood/body fluid analysis; Criminal investigation; Criminalistics; DNA fingerprinting; Evidence identification; Forensic medicine; Forensic sciences; Minnesota; Saliva sample analysis; Tissue analysis; Urinalysis
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