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NCJRS Abstract

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NCJ Number: 168689 Find in a Library
Title: Enantioselective Separation of Methadone and Its Main Metabolite in Human Hair by Liquid Chromatography/Ion Spray-Mass Spectrometry
Journal: Journal of Forensic Sciences  Volume:42  Issue:2  Dated:(March 1997)  Pages:291-295
Author(s): P Kintz; H P Eser; A Tracqui; M Moeller; V Cirimele; P Mangin
Date Published: 1997
Page Count: 5
Sponsoring Agency: Office of National Drug Control Policy
Washington, DC 20500
Contract Number: N00014-95-k-2000
Type: Test/Measurement
Format: Article
Language: English
Country: United States of America
Annotation: This paper describes a method for the stereoselective determination of both enantiomers of methadone and 2-ethyl-1,5- dimethyl-3,3-diphenylpyrrolinium (EDDP) in human hair, using an alpha1-acid glycoprotein (Chiral-AGP) HPLC column and mass spectrometric detection.
Abstract: Optical isomers exhibit significant differences in their affinities for receptor sites, biotransformation, and binding to serum and tissue proteins. Methadone has been used for the substitution of heroin addicts since 1964. The racemic form is used, that is, a mixture of the biologically active R-form and the practically inactive S-form. To investigate methadone distribution, a chiral separation of the isomers was developed in human hair samples. The method involves decontamination of hair with water and acetone, pulverization in a ball mill, enzymatic hydrolysis in the presence of deuterated internal standards, solid-phase extraction, and liquid chromatography/ion spray-mass spectrometry. Enantioselective separation of methadone and its main metabolite, EDDP, was obtained using an alpha1-acid glycoprotein column (100 by 4 mm ID). In all nine specimens obtained from subjects under racemic methadone treatment in a detoxification center, R- and S-enantiomers of methadone and EDDP were identified with the following concentrations: 2.58-10.22, 1.89-9.53, 0.42-1.73, and 0.40-2.10 ng/mg for R-methadone, S- methadone, R-EDDP, and S-EDDP, respectively. Results are suggestive of a predominance of the R-enantiomer of methadone in human hair. 3 figures, 2 tables, and 17 references
Main Term(s): Drug analysis
Index Term(s): Hair and fiber analysis; Methadone
Note: DCC.
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