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NCJ Number: 183294 Find in a Library
Title: Blood Grouping of Mixed Bloodstains Using Immunocytochemical Methods
Journal: Journal of Forensic Sciences  Volume:44  Issue:1  Dated:January 1999  Pages:100-104
Author(s): Yasuo Bunai Ph.D.; Isao Nakamura; Atsushi Nagai Ph.D.; Sadao Yamada Ph.D.; Yoshihisa Watanabe M.A.; Tomohiro Takayama B.A.; Isao Ohya Ph.D.
Date Published: January 1999
Page Count: 5
Type: Report (Study/Research)
Format: Article
Language: English
Country: United States of America
Annotation: This paper describes immunocytochemical methods for determining the ABO blood group of each blood of mixed bloodstains; these methods include the peroxidase-anti-peroxidase (PAP) and the labeled streptavidin-biotin (LSAB) methods.
Abstract: The research took place in Japan. Mixed bloodstains were made on surgical blades and a cedar board. The blades were dipped into of one sample of blood and then dipped into blood of a different group at intervals of 30, 20, 15, 10, and 5 seconds. Two drops of blood were dropped on a cedar board; two drops of a different group were dropped there at the same intervals. The bloodstains dried for a week. The blood samples were removed from the blades or the cedar board and processed with a routine histological method. Three serial thin sections were obtained. The sections were deparaffinized and treated in papain solution for 2 hours at 36 degrees centigrade to unmask antigenic sites on red cell membranes. The research used the LSAB and PAP methods to detect A and B antigens and an indirect immunocytochemical method for H antigen. Results of these methods revealed specific immunologic reactions and allowed determination of the blood group of each blood of mixed bloodstains. Furthermore, these methods indicated the possibility of determining who was stabbed first in situations in which a single knife stabbed two or more victims. Figures, table, and 8 references (Author abstract modified)
Main Term(s): Foreign police
Index Term(s): Blood stains; Blood/body fluid analysis; Japan; Scientific techniques; Suspect identification; Victim identification
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