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NCJ Number: 183300 Find in a Library
Title: Deoxyribonuclease I Phenotyping From Saliva Stains
Journal: Journal of Forensic Sciences  Volume:44  Issue:1  Dated:January 1999  Pages:175-178
Author(s): Reiko Iida Ph.D.; Kazumi Sawazaki Ph.D.; Toshihiro Yasuda Ph.D.; Etsuko Tsubota; Masahiro Aoyama M.D.; Takasumi Matsuki M.D.; Koichiro Kishi M.D.
Date Published: January 1999
Page Count: 4
Type: Research (Applied/Empirical)
Format: Article
Language: English
Country: United States of America
Annotation: This paper describes a newly developed method for extraction and purification of DNase I from saliva stains.
Abstract: The research took place in Japan. DNase I phenotyping from saliva stains had previously been unsuccessful due to low enzyme activity and heavy contamination. The present research extracted salivary DNase I from stains using phosphate bufer containing Nonidet P-40. Extracts were purified using Phenyl Sepharose CL-4B gel. Electrophoresis was performed, and DNase I was successfully phenotyped. All the DNase I phenotypes obtained from saliva stains using this new method were identical to the phenotypes determined from urine samples. Moreover, this method correctly phenotyped DNase I from saliva stains that had been stored for more than 3 months at room temperature or at 37 degrees centigrade. Findings suggested that DNase I polymorphisms provide useful information for forensic characterization of saliva stains. Figures, table, and 18 references (Author abstract modified)
Main Term(s): Science and Technology
Index Term(s): DNA fingerprinting; Evidence identification; Forensic medicine; Japan; Saliva sample analysis; Scientific techniques; Suspect identification; Urinalysis; Victim identification
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