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NCJRS Abstract

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NCJ Number: 210791 Find in a Library
Title: U.S. Population Data for the Multi-Copy Y-STR Locus DYS464
Journal: Journal of Forensic Sciences  Volume:50  Issue:4  Dated:July 2005  Pages:975-977
Author(s): John M. Butler Ph.D.; Richard Schoske Ph.D.
Date Published: July 2005
Page Count: 3
Type: Report (Study/Research) ; Test/Measurement
Format: Article
Language: English
Country: United States of America
Annotation: This paper reports on the amplification of the multi-copy tetranucleotide Y-chromosome short tandem repeat (Y-STR) marker DYS464 for 679 male U.S. population samples as part of a multiplex Y-STR assay.
Abstract: The samples obtained included 262 Caucasians, 265 African-Americans, and 152 Hispanics. Amplification was done with the following PCR primers: VIC-CTTTGGGCTATGCCTCAGTTT and GCCATACCTGGGTAACAGAGAGAC. This set of primers target at least 4 separate regions of the Y-chromosome and can generate up to 4 distinct peaks in the size range of 242-286 bp that have an allele range of 9-20 CCTT repeats. A table presents a summary of the haplotypes observed based on peak height information with the DYS464 locus. Across the 679 samples examined, 179 different expanded types were observed, with 92 occurring only once. If the information presented in the table is collapsed to include only the peaks observed (no consideration of the relative peak heights), then 113 conservative types are evident. In 13 individuals, there were apparently more than 4 copies of DYS464 present, based on the allele peak heights. In addition, 10 samples appeared to have a deletion of 1 of the copies. This phenomenon of Y-chromosomal duplication and deletion has been observed with other Y-STR markers used in human identity testing. The authors advise that DYS464 is a valuable, highly polymorphic marker that can be useful in paternity testing and genetic genealogy applications. 1 table and 8 references
Main Term(s): Police policies and procedures
Index Term(s): Black/African Americans; Caucasian/White Americans; DNA fingerprinting; Forensic sciences; Hispanic Americans; Investigative techniques
Note: The complete dataset with DYS464 and other human identity testing markers on these U.S. samples is available at
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