skip navigation


Register for Latest Research

Stay Informed
Register with NCJRS to receive NCJRS's biweekly e-newsletter JUSTINFO and additional periodic emails from NCJRS and the NCJRS federal sponsors that highlight the latest research published or sponsored by the Office of Justice Programs.

NCJRS Abstract

The document referenced below is part of the NCJRS Virtual Library collection. To conduct further searches of the collection, visit the Virtual Library. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.


NCJ Number: 211161 Find in a Library
Title: Obtaining Typable DNA From Bloodstains That Serologically Test Negative
Journal: Journal of Forensic Identification  Volume:55  Issue:5  Dated:September/October 2005  Pages:633-643
Author(s): Katie L. Coy; Kristen E. Lewis; Ashlee Fulmer; Amy Hudson; Tracey Dawson Cruz
Date Published: September 2005
Page Count: 11
Type: Research (Applied/Empirical)
Format: Article
Language: English
Country: United States of America
Annotation: This study evaluated whether typable DNA could be obtained from bloodstains treated with household detergents and bleach, specifically when a negative serological result was obtained.
Abstract: Prior research studies have reported conflicting results regarding the effects of bleach and detergents on the analysis of bloodstained material. This study investigated the effects of different washing treatments on the whole bloodstains of varying concentrations. It is known that certain washing conditions can lead to false negative serological results, which are potentially due to the denaturing properties of these conditions. Three sets of bloodstains were created by making dilutions of whole blood with sterile water. Each sample was tested using the ABAcard HemaTrace confirmatory blood test. The untreated-washed whole bloodstain, untreated-washed 1:20 dilution, and the whole bloodstain washed in Tide were the only samples to produce a positive reading for human hemoglobin with the confirmatory blood test. The data produced indicate that confirmatory serology tests for blood are not always reliable predictors of successful STR amplification. Investigators and examiners should use caution when interpreting screening test results, because negative serological results cannot always be used to predict the ability to successfully genotype DNA. Table, figures, references
Main Term(s): DNA fingerprinting
Index Term(s): Blood stains; Blood/body fluid analysis; Evidence identification; Forensic sciences
To cite this abstract, use the following link:

*A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's website is provided. Tell us how you use the NCJRS Library and Abstracts Database - send us your feedback.