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NCJ Number: 211285 Find in a Library
Title: Analysis of Non-Suspect Samples Lacking Visually Identifiable Sperm Using a Y-STR 10-Plex
Journal: Journal of Forensic Sciences  Volume:50  Issue:5  Dated:September 2005  Pages:1116-1118
Author(s): Cassie L. Johnson M.S.; Robert C. Giles Ph.D.; Joseph H. Warren B.A.; Judith I. Floyd B.S.; Rick W. Staub Ph.D.
Date Published: September 2005
Page Count: 3
Type: Research (Applied/Empirical)
Format: Article
Language: English
Country: United States of America
Annotation: This study examined the effectiveness of Y-chromosome short tandem repeats (Y-STRs) where there was absence of visually identifiable sperm in non-suspect samples.
Abstract: Y-chromosome short tandem repeats (Y-STRs) are important in forensic testing because of their ability to genetically identify the male component of a sample. More specifically, they are valuable in the investigation of sexual assaults in which autosomal STR genotype interpretation is challenging. In sexual assault samples, the amount of female DNA overwhelms the quantity of male DNA present. So, the true genotype of the male suspect can be masked by the female victim’s profile, making interpretation difficult. Utilizing the Y-STR 10 plex, this study examined and determined the effectiveness of Y-chromosome STRs in profiling male DNA from non-suspect sexual assault samples lacking visually identifiable spermatozoa. To determine male DNA from compromised sexual assault evidence, 45 non-suspect samples were differentially extracted and analyzed. The study indicates that it is possible to obtain a male STR profile even in the absence of visually identifiable spermatozoa and that Y-STR loci should become components of CODIS to be used in solving non-suspect sexual assaults. Table, references
Main Term(s): DNA fingerprinting
Index Term(s): Blood/body fluid analysis; Evidence identification; Forensic sciences; Sexual assault; Suspect identification
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