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NCJ Number: 218475 Find in a Library
Title: Forensic Mitochondrial DNA Analysis of 116 Casework Skeletal Samples
Journal: Journal of Forensic Sciences  Volume:52  Issue:3  Dated:May 2007  Pages:557-561
Author(s): Kimberlyn Nelson Ph.D.; Terry Melton Ph.D.
Date Published: May 2007
Page Count: 5
Type: Report (Study/Research)
Format: Article
Language: English
Country: United States of America
Annotation: In order to assess the National Missing Person DNA Database Program's lab in identifying skeletal remains, this study retrospectively examined the lab's ability to develop mitochondrial DNA (mtDNA) profiles from the remains submitted to the lab in the last 6 1/2 years.
Abstract: The study found that at least a partial mtDNA profile was obtained for 83.6 percent of the samples. There were two general categories of skeletal remains: samples submitted for body identifications by law enforcement agencies and samples submitted to determine historical origins or family identity. Body identification cases were more likely to yield full mtDNA profiles; whereas, historical cases were more likely to result in partial profiles. The ability to obtain a full or partial mtDNA profile was related primarily to the difference in the average age and condition of the samples in the two case categories. Cremated remains uniformly failed to produce mtDNA profiles. Infant/fetal remains, on the other hand, uniformly yielded mtDNA profiles. Mitochondrial DNA profiles were generated from bones and teeth submitted for casework. This consisted of 116 DNA extractions completed on skeletal remains from routine casework conducted between February 1999 and May 2005. Samples were processed individually and extracted in parallel with a reagent blank control that accompanied the sample throughout testing. This report provides detailed descriptions of DNA extraction and PCR amplification and sequencing. 2 tables, 2 figures, and 23 references
Main Term(s): Police policies and procedures
Index Term(s): DNA fingerprinting; Evidence collection; Forensic sciences; Missing children; Missing person investigation; Victim identification
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