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NCJ Number: 219252 Find in a Library
Title: Concordance Study Between the AmpFlSTR MiniFiler PCR Amplification Kit and Conventional STR Typing Kits
Journal: Journal of Forensic Sciences  Volume:52  Issue:4  Dated:July 2007  Pages:870-873
Author(s): Carolyn R. Hill M.S.; Margaret C. Kline M.S.; Julio J. Mulero Ph.D.; Robert E. Lagace B.A.; Chien-Wei Chang Ph.D.; Lori K. Hennessy Ph.D.; John M. Butler Ph.D.
Corporate Author: National Institute of Standards and Technology (NIST)
United States of America
Date Published: July 2007
Page Count: 4
Sponsoring Agency: National Institute of Justice (NIJ)
Washington, DC 20531
National Institute of Standards and Technology (NIST)
Gaithersburg, MD 20899-3460
Grant Number: 2003-IJ-R-029
Type: Test/Measurement
Format: Article
Language: English
Country: United States of America
Annotation: This paper reports on a "concordance" (agreement or harmony) study that compared the DNA results for 1,308 samples evaluated with both the AmpFlSTR MiniFiler and Identifiler STR kits.
Abstract: Full concordance between Identifiler and MiniFiler kits was found in 99.7 percent (10,437 out of 10,464) STR allele calls compared. The 27 differences found encompassed the loci D13S317 (n=14) and D16S539 (n=10) as well as D18S51 (n=1), D7S820 (n=1), and CSF1PO (n=1). The other three STR loci, D2S1338, FGA, and D21S11, and the sex determining locus amelogenin were fully concordant at all samples examined in this study. Three of the null alleles detected in the study were from children of fathers who also possessed the mutation that impacted the primer-binding site. Genotyping discrepancies between the Identifiler and MiniFiler kits were confirmed in 17 cases by reamplification of the samples and further testing using the PowerPlex 16 kit. DNA sequence analysis was also performed in order to determine the nature of the genetic variations that caused the allele dropout or apparent repeat unit shift. The 1,308 samples tested included 449 African-Americans, 445 Caucasians, 207 Hispanics, and 207 Asians. 1 table, 2 figures, and 9 references
Main Term(s): Police policies and procedures
Index Term(s): Comparative analysis; Crime laboratory equipment; DNA fingerprinting; Forensic sciences; Investigative techniques; NIJ grant-related documents
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