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NCJRS Abstract

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NCJ Number: 220411 Find in a Library
Title: Validation of Reduced-Scale Reactions for the Quantifiler Human DNA Kit
Journal: Journal of Forensic Sciences  Volume:52  Issue:5  Dated:September 2007  Pages:1035-1043
Author(s): Christian G. Westring B.S.; Richard Kristinsson M.S.; Dustin M. Gilbert B.S.; Phillip B. Danielson Ph.D.
Date Published: September 2007
Page Count: 9
Sponsoring Agency: National Institute of Justice (NIJ)
Washington, DC 20531
National Science Foundation
Arlington, VA 22230
Grant Number: 2003-IJ-CX-K104;0200484
Type: Report (Technical); Test/Measurement
Format: Article
Language: English
Country: United States of America
Annotation: This study evaluated the performance of reduced-volume (10 ml) DNA quantification assays with the use of the Quantifiler Human DNA Quantification Kit, using commercial standards and single-source biological stains (e.g., venous blood, saliva, and semen), as well as casework-type samples that included those subjected to environmental contaminants and samples with extensive DNA degradation.
Abstract: The study demonstrated the same performance between the 25 ml and 10 ml quantifiler reaction volumes. This reduction in reaction volume has the practical benefit of increasing the effective number of reactions per kit by 250 percent. This reduces the cost per assay by 60 percent while consuming less sample. The amplification efficiency and reproducibility of reduced-scale reactions were found to be virtually indistinguishable or to trend slightly better than the manufacturer's recommended protocol for amplification of a broad range of dilutions of the commercially prepared human DNA standard provided with the Quantifiler kit. The same equivalence in performance was achieved with single-source blood samples extracted from cotton swabs. With more challenging mixtures of blood and top soil, the reduced-scale reactions produced equivalent to slightly more precise quantification results than full-scale assays of the same DNA extract. Reduced-scale reactions were used to quantify the DNA content of a variety of simulated casework samples and postmortem blood samples that contained highly degraded DNA. The description of materials and methods addresses DNA sample preparation and extraction, quantitative real-time PCR, and STR genotyping. 3 tables, 7 figures, and 29 references
Main Term(s): Criminology
Index Term(s): Crime laboratory equipment; DNA fingerprinting; Equipment evaluation; Forensic sciences; Instrument validation; Investigative techniques; NIJ grant-related documents; Suspect identification; Victim identification
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