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NCJRS Abstract

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NCJ Number: 221661 Find in a Library
Title: mRNA Profiling for Body Fluid Identification by Multiplex Quantitative RT-PCR
Journal: Journal of Forensic Sciences  Volume:52  Issue:6  Dated:November 2007  Pages:1252-1262
Author(s): Jane Juusola Ph.D.; Jack Ballantyne Ph.D.
Date Published: November 2007
Page Count: 11
Sponsoring Agency: US Federal Bureau of Investigation
Washington, DC, 20535
Grant Number: J-FBI-03-287
Type: Report (Study/Research)
Format: Article
Language: English
Country: United States of America
Annotation: This study developed sensitive and robust multiplex quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) assays for the identification of blood, saliva, semen, and menstrual blood.
Abstract: The study found that the qRT-PCR assays used are 5-fold to 40-fold more sensitive than a capillary electrophoresis (CE) analysis for body fluid identification. Specificity is maintained over a wide range of RNA input concentrations (20 fg-500 ng). Normalization of both body fluid-specific genes to the housekeeping gene GAPDH by means of appropriate cycle threshold metrics ensures the high specificity of each assay for its cognate body fluid. The body fluid-specific genes included crythroid delta-aminolevulinate synthase (ALAS2) and beta-spectrin (SPTB) for blood, statherin (STATH) and histatin 3 (HTN3) for saliva, protamine 1 (PRM1) and protamine 2 (PRM2) for semen, and matrix metalloproteinase 7 (MMP7) and matrix metalloproteinase 10 (MMP10) for menstrual blood. Body fluids were collected from healthy individuals. The description of materials and methods addresses body fluid samples, RNA isolation and quantitation, cDNA synthesis, standard end-point PCR, real-time PCR primer and probe design, and real-time PCR. 2 tables, 5 figures, and 17 references
Main Term(s): Police policies and procedures
Index Term(s): Blood/body fluid analysis; DNA fingerprinting; Forensic sciences; Investigative techniques; Suspect identification
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