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NCJ Number: 221663 Find in a Library
Title: Fate of the Chemical Warfare Agent During DNA Extraction
Journal: Journal of Forensic Sciences  Volume:52  Issue:6  Dated:November 2007  Pages:1272-1283
Author(s): Della A. Wilkinson Ph.D.; Albert G. Hulst B.Sc.; Leo P.J. de Reuver B.Sc.; Simon H. van Krimpen B.Sc.; Ben M.L. van Baar Ph.D.
Date Published: November 2007
Page Count: 12
Sponsoring Agency: Royal Canadian Mounted Police
Ottawa, Ontario K1A 0R2, Canada
US Dept of Homeland Security
Washington, DC 20528
Type: Report (Study/Research)
Format: Article
Language: English
Country: United States of America
Annotation: This study examined the fate of four toxic chemical warfare agents--sulfur mustard, sodium 2-fluoracetate, sarin, and diazinon--in a lysis buffer used in Promega DNA IQ extraction, in order to determine whether extraction would render the samples safe.
Abstract: Agent levels in the elution buffer were found to be below the detectable limits for mustard, sarin, sodium 2-fluoroacetate or low (less than 0.02 mg/ml) for diazinon; therefore, once extracted, these DNA samples could be safely processed in a forensic laboratory. The authors note, however, that these analyses were not conducted with real DNA samples, but rather with neat buffer. Different chemical-warfare-agent behavior may occur in a real-world situation. It is known that salt content (ionic strength) and pH influence chemical agent degradation. As chemical containment would be required for the processing of samples contaminated at levels of 10 mg/ml of sarin, diazinon, or sodium 2-fluoroacetate, processing of samples similarly contaminated with sulfur mustard would be best also done in chemical containment in order to maintain a consistent protocol. Two independent analytical methods were used per agent, selected from GC-MS, H NMR, F NMR, P NMR, or LC-ES MS. The methods were validated before use. Determinations were performed in a semi-quantitative way by direct comparison to standards. Materials and methods are described in detail. 8 tables, 10 figures, and 15 references
Main Term(s): Police policies and procedures
Index Term(s): Chemical Weapons; Crime laboratories; DNA fingerprinting; Forensic sciences; Investigative techniques; Occupational safety and health; Victim identification
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