skip navigation


Register for Latest Research

Stay Informed
Register with NCJRS to receive NCJRS's biweekly e-newsletter JUSTINFO and additional periodic emails from NCJRS and the NCJRS federal sponsors that highlight the latest research published or sponsored by the Office of Justice Programs.

NCJRS Abstract

The document referenced below is part of the NCJRS Virtual Library collection. To conduct further searches of the collection, visit the Virtual Library. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.


NCJ Number: 222151 Find in a Library
Title: Characterization of 26 MiniSTR Loci for Improved Analysis of Degraded DNA Samples
Journal: Journal of Forensic Sciences  Volume:53  Issue:1  Dated:January 2008  Pages:81-89
Author(s): Carolyn R. Hill M.S.; Margaret C. Kline M.S.; Michael D. Coble Ph.D.; John M. Butler Ph.D.
Date Published: January 2008
Page Count: 9
Sponsoring Agency: National Institute of Justice (NIJ)
Washington, DC 20531
Grant Number: 2003-IJ-R-029
Type: Report (Study/Research)
Format: Article
Language: English
Country: United States of America
Annotation: This study characterized 26 new loci (20 in addition to the 6 previously developed by the Biochemical Science Division of the National Institute of Standards and Technology) in order to improve the analysis of degraded DNA samples.
Abstract: All but 1 locus of the additional 20 mini-short tandem repeat (miniSTR) markers was under 140 base pairs (bp). These new loci were run across 665 U.S. population samples, including African-Americans, Caucasians, and Hispanics. Population statistics were determined for each group. All 665 samples were resolved from one another with all 26 new loci. The heterozygosity values for all of these markers were comparable to those of the 13 core CODIS loci, with a few of the new marker values being lower. Allelic ladders were created for each of these new miniSTR loci using the population samples. Now that each of these miniSTR loci have been characterized and optimized, the goal is to create larger multiplexes, with several loci present in different dye sets, so as to maximize the effectiveness of these markers on reference samples. Even used as single markers, however, their contribution to the forensic community in assisting in the analysis of degraded DNA samples may prove useful, as well as providing additional discrimination in complex paternity cases or missing person cases. Many of these miniSTR markers are being currently used in laboratories across the world as supplements in order to obtain full profiles from samples that generate partial profiles using commercial STR kits. Descriptions of materials and methods address the selection of loci and primer design, the source of DNA samples for population testing, PCR amplification, analysis on the ABI 3100 and 3130xl Genetic Analyzer, the generation of allelic ladders and GeneMapper ID bins and panels, DNA sequencing, and data analysis. 5 tables, 2 figures, and 22 references
Main Term(s): Criminology
Index Term(s): DNA fingerprinting; Forensic sciences; Investigative techniques; NIJ grant-related documents; Suspect identification; Victim identification
To cite this abstract, use the following link:

*A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's website is provided. Tell us how you use the NCJRS Library and Abstracts Database - send us your feedback.