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NCJ Number: 222202 Find in a Library
Title: Evaluation of Haplotype Discrimination Capacity of 35 Y-Chromosomal Short Tandem Repeat Loci
Journal: Forensic Science International  Volume:174  Issue:2-3  Dated:January 2008  Pages:182-188
Author(s): Heike Rodig; Lutz Roewer; Annett Gross; Tom Richter; Peter de Knijff; Manfred Kayser; Werner Brabetz
Date Published: January 2008
Page Count: 7
Sponsoring Agency: German Federal Ministry of Economics and Technology (BMWI)
11019 Berlin,
Grant Number: IW041368
Type: Report (Study/Research)
Format: Article
Language: English
Country: United States of America
Annotation: In order to identify the minimum number of Y-STRs (Y-chromosomal short tandem repeat loci) needed for individualizing male lineages, in addition to the recommended 9 minimal haplotype loci or the 11 SWGDAM (Scientific Working Group on DNA Analysis Methods) loci, this study assessed the haplotype discrimination capacity of the 9 Y-STR loci comprising the minimal haplotype, together with an additional 26 recently described single-copy Y-STRs, for 391 males from Germany, the Netherlands, and Turkey.
Abstract: The study showed that the typing of five Y-STRs, in addition to the nine minimal haplotype loci, revealed complete haplotype resolution and thus complete individualization of all male lineages in each of the eight population samples analyzed. In the pooled dataset of 391 males, 7 Y-STRs in addition to the 9 minimal haplotype loci revealed complete individualization. The five Y-STRs DYS447, DYS449, DYS481, DYS570, and DYS576 composed the smallest set of loci in addition to the previously recommended standard Y-STRs leading to the individualization of all males from each single population group. Complete resolution of the pooled population was achieved by the additional genotyping of two additional loci, DYS446 or DYS505 and DYF406S1 or DYS522. DNAs from unrelated persons of eight populations were used for the evaluation of the Y-STR loci. The German populations were Berlin (n=55), Sorbs (n=29), Munich (n=30), Hamburg (n=49), Dresden (n=80), and Rostock (n=36). In addition, a Dutch population of Leiden (n=72) and a Turkish population living in Berlin (n=40) were analyzed. For the amplification of the Y-STR loci, eight multiplex PCR systems were used. 5 tables and 21 references
Main Term(s): Criminology; Foreign countries
Index Term(s): DNA fingerprinting; Gender determination; Gender issues; Germany; Investigative techniques; Netherlands; Suspect identification; Turkey
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