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NCJRS Abstract

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NCJ Number: 222204 Find in a Library
Title: Analysis of Ketamine and Norketamine in Urine by Automatic Solid-Phase Extraction (SPE) and Positive Ion Chemical Ionization-Gas Chromatography-Mass Spectrometry (PCI-GC-MS)
Journal: Forensic Science International  Volume:174  Issue:2-3  Dated:January 2008  Pages:197-202
Author(s): Eun-mi Kim; Ju-seon Lee; Sang-kil Choi; Mi-ae Lim; Hee-sun Chung
Date Published: January 2008
Page Count: 6
Type: Report (Study/Research)
Format: Article
Language: English
Country: United States of America
Annotation: This study assessed a method for identifying ketamine (KT) and its major metabolite, norketamine (NK), in urine, using positive ion chemical ionization-gas chromatography-mass spectrometry (PCI-GC-MS) with an automatic solid-phase extraction (SPE) apparatus.
Abstract: According to the validation data for this method, the analysis of urine by SPE and PCI-GC-MS is suitable for the detection and quantification of KT in human urine. Extracted calibration curve of KT and NK were linear from 50 to 1000 ng/mL with correlation coefficient 0.99. The limit of quantitation of KT and NK was the first point of the calibration curve, 50 ng/mL, and the limits of detection, which were defined as the detection limits of the target and qualifier ion peaks on each mass chromatogram, was estimated to be 25 ng/mL in the full-scan modes. The values of accuracy for KT and NK at three different concentration levels were less than 15 percent. SPE is simple and requires less organic solvent than liquid-liquid extraction, and PCI-GC-MS can offer both qualitative and quantitative information for urinalysis of KT. The analytical method was applied to six KT suspected urine specimens and KT-administered rat urine, and the concentrations of KT and NK were determined. 3 tables, 7 figures, and 7 references
Main Term(s): Criminology
Index Term(s): Chromatography; Drug testing; Mass spectroscopy; Urinalysis
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