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NCJRS Abstract

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NCJ Number: 222339 Find in a Library
Title: Recovery and Stability of RNA in Vaginal Swabs and Blood, Semen, and Saliva Stains
Journal: Journal of Forensic Sciences  Volume:53  Issue:2  Dated:March 2008  Pages:296-305
Author(s): Mindy Setzer M.S.; Jane Juusola Ph.D.; Jack Ballantyne Ph.D.
Date Published: March 2008
Page Count: 10
Sponsoring Agency: University of Central Florida
Orlando, FL 32816
Type: Report (Study/Research)
Format: Article
Language: English
Country: United States of America
Annotation: This study determined the persistence and stability of RNA (mRNA), the molecular intermediate between DNA and protein that is expressed in a tissue-specific manner, in stains prepared from blood, saliva, semen, and vaginal secretions that were exposed to a range of environmental conditions from 1 to 547 days.
Abstract: The study demonstrated that total RNA can be recovered from biological stains exposed to a variety of different environmental conditions in sufficient quantities to perform mRNA-based assays. On average, several hundred nanograms of total RNA were recovered from 50-ml-sized blood and saliva stains, 1 mg from a 50-ml semen stains, and nearly 70 mg from a whole vaginal swab. Messenger RNA was detectable in some samples stored at room temperature for at least 547 days. The environmental samples that were protected from direct rain impact exhibited housekeeping and tissue-specific mRNA recoverability up to 7 days for saliva and semen, 30 days for blood, and 180 days for the vaginal swab. Rain had a detrimental effect on the recoverability of specific transcripts for blood (3 days), saliva (1 day), semen (7 days), and vaginal secretions (3 days). The semen marker PRM2 was not detectable after 1 day. The persistence and stability of RNA within each type of body fluid stain were determined by quantitation of total RNA and reverse transcriptase-polymerase chain reaction, using eight different mRNA transcripts from selected housekeeping and tissue-specific genes. The description of material and methods addresses the body fluid samples RNA isolation, DNase treatment, RNA quantitation, cDNA synthesis, polymerase chain reaction, and gel electrophoresis. 3 tables, 4 figures, and 25 references
Main Term(s): Blood/body fluid analysis; Criminology; Florida
Index Term(s): DNA fingerprinting; Forensic sciences; Investigative techniques; Saliva sample analysis; Suspect identification
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