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NCJRS Abstract

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NCJ Number: 222344 Find in a Library
Title: Direct STR Amplification From Whole Blood and Blood- or Saliva-Spotted FTA Without DNA Purification
Journal: Journal of Forensic Sciences  Volume:53  Issue:2  Dated:March 2008  Pages:325-341
Author(s): Su Jeong Park Ph.D.; Jong Yeol Kim M.S.; Young Geun Yang Ph.D.; Seung Hwan Lee Ph.D.
Date Published: March 2008
Page Count: 7
Sponsoring Agency: Korea Science and Engineering Foundation (KOSEF)
Daejeon 305-350, Korea
Ministry of Science and Technology (MOST)
Seoul, Republic of Korea 110-760,
Grant Number: M10640010002-06N4001-00210
Type: Report (Study/Research)
Format: Article
Language: English
Country: United States of America
Annotation: This report describes a new method for direct short tandem repeat (STR) amplification that uses a newly developed direct polymerase chain reaction (PCR) buffer, AnyDirect, which can amplify STR loci from whole blood and blood-spotted or saliva-spotted FTA cards without DNA purification.
Abstract: Regarding concordance with purified DNA, all DNA profiles from blood and blood spots from 50 individuals matched fully; however, regarding saliva spots, poor amplifications were sometimes observed, suggesting that this issue should be studied further. This system had broad compatibility with various DNA polymerase and STR kits. The report also concludes that direct PCR can be applied to old spots, which indicates that the system should be useful for fast DNA typing when there is no need to extract and store DNA, as well as when high-throughput is required. In order to be used actively in forensic case work, further validation studies are required for various kinds of forensic stains and materials that might contain strong PCR inhibitory factors. The autosomal and Y chromosomal STR loci were analyzed for whole blood or saliva spots from 50 random volunteers followed by comparison of the results with those of corresponding purified DNA. In addition to a description of blood and saliva specimens, the description of materials and methods addresses STR amplifications and fragment analysis. The AnyDirect PCR buffer is composed mainly of zwitterionic buffer and/or nonreducing carbohydrates, so as to overcome the PCR inhibitory effects and dNTP's. 3 tables, 5 figures, and 10 references
Main Term(s): Blood/body fluid analysis; Criminology; Korea (South)
Index Term(s): Blood stains; DNA fingerprinting; Foreign criminal justice research; Saliva sample analysis
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