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NCJRS Abstract

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NCJ Number: 222347 Find in a Library
Title: Dual Examinations for Identification of Urine as Being of Human Origin and for DNA-Typing From Small Stains of Human Urine
Journal: Journal of Forensic Sciences  Volume:53  Issue:2  Dated:March 2008  Pages:359-363
Author(s): Takehiko Nakazono Ph.D.; Seiich Kashimura Ph.D.; Yasuhiko Hayashiba Ph.D.; Kenji Hara Ph.D.; Aya Matsusue M.S.; Christa Augustin Ph.D.
Date Published: March 2008
Page Count: 5
Type: Report (Study/Research)
Format: Article
Language: English
Country: United States of America
Annotation: This study examined concurrent methods for the identification of urine as being of human origin and for DNA-typing from small stains of human urine.
Abstract: The study found a means of unifying two tests, one for identifying urine as of human origin and a second for DNA typing from small stains of human urine, for use on just one sample. A urine stain was extracted with phosphate-buffered saline, and the extract was filtered using a Centricon-100 device. The filtrate was subjected to electrospray ionization liquid chromatography-mass spectrometry for identification of human urine, and a DNA-typing sample was obtained by dialfiltration of the residue, using a DNA purification kit. After the purified residue was treated with an AmpflSTR Profiler PCR amplification kit, the DNA-types were analyzed by capillary electrophoresis using a Genetic analyzer. This procedure was successfully applied in a murder case that involved a urine stain found on a waterproof sheet in a motel bedroom. Detailed descriptions of materials and methods address the reagents used, standard solutions, sample characteristics and preparation, the extraction procedure, conditions of high-performance liquid chromatography/mass spectrography, and amplification and electrophoresis of DNA. 1 table, 6 figures, and 4 references
Main Term(s): Blood/body fluid analysis; Criminology; Japan
Index Term(s): DNA fingerprinting; Foreign criminal justice research; Forensic sciences; Investigative techniques
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