skip navigation

PUBLICATIONS

Register for Latest Research

Stay Informed
Register with NCJRS to receive NCJRS's biweekly e-newsletter JUSTINFO and additional periodic emails from NCJRS and the NCJRS federal sponsors that highlight the latest research published or sponsored by the Office of Justice Programs.

NCJRS Abstract

The document referenced below is part of the NCJRS Virtual Library collection. To conduct further searches of the collection, visit the Virtual Library. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.

 

NCJ Number: 228341 Find in a Library
Title: Tri-Allelic SNP Markers Enable Analysis of Mixed and Degraded DNA Samples
Journal: Forensic Science International: Genetics  Volume:3  Issue:4  Dated:September 2009  Pages:233-241
Author(s): Antoinette A. Westen; Anuska S. Matai; Jeroen F.J. Laros; Hugo C. Meiland; Mandy Jasper; Wiljo J.F. de Leeuw; Peter de Knijff; Titia Sijen
Date Published: September 2009
Page Count: 9
Publisher: http://www.elsevier.com 
Type: Report (Study/Research)
Format: Article
Language: English
Country: Ireland
Annotation: This article reports on the development of an algorithm to find nonbinary (i.e., tri-allelic or tetra-allelic) single nucleotide polymorphisms (SNPs) in the NCBI dbSNP database.
Abstract: The study concluded that tri-allelic SNP markers are suited for the analysis of degraded DNA and enable the detection of a second DNA source in a sample. Fifteen tri-allelic SNPs on 14 different chromosomes were detected in DNA samples from Dutch and Netherlands Antilles donors. The study showed that such nonbinary SNPs have the ability to reveal the presence of a second DNA donor in mixed samples with a ratio of up to 1:8. Indications for a mixture are the presence of a third allele on one locus, unexpected peak height ratios, and uncalled peaks above the detection threshold. Several of the tri-allelic SNP markers may not only be useful for identification purposes, but also as ancestry informative markers. In addition, degraded (UV irradiated, TURBO DNase treated, and 500-year-old bone and molar) DNA samples show that when the higher molecular weight STR markers fail to amplify, tri-allelic SNP markers can still provide valuable information. Multiplex SNaPshot assays were developed, and the allele frequencies of 16 SNPs were determined for 152 Dutch and 111 Netherlands Antilles reference samples. The descriptions of materials and methods address sample characteristics, SNP selection, PCR, SNaPshot (single base extension), SNP detection and analysis, allele frequencies and statistics, and sequencing. 2 tables, 4 figures, and 35 references
Main Term(s): Criminology
Index Term(s): DNA fingerprinting; Evidence identification; Forensic sciences; Investigative techniques; Suspect identification
To cite this abstract, use the following link:
http://www.ncjrs.gov/App/publications/abstract.aspx?ID=250360

*A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's website is provided. Tell us how you use the NCJRS Library and Abstracts Database - send us your feedback.