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NCJ Number: 229678 Find in a Library
Title: Study of PCR Inhibition Mechanisms Using Real Time PCR
Journal: Journal of Forensic Sciences  Volume:55  Issue:1  Dated:January 2010  Pages:25-33
Author(s): Kerry L. Opel, Ph.D.; Denise Chung, Ph.D.; Bruce R. McCord, Ph.D.
Date Published: January 2010
Page Count: 9
Sponsoring Agency: National Institute of Justice (NIJ)
Washington, DC 20531
Grant Number: 2006-DN-BX-K006
Publisher: http://www3.interscience.wiley.com 
Type: Report (Study/Research)
Format: Article
Language: English
Country: United States of America
Annotation: In this project, real time polymerase chain reaction (PCR) was utilized to study the mechanism of PCR inhibition through examination of the effect of amplicon length, melting temperature, and sequence.
Abstract: Specifically designed primers with three different amplicon lengths and three different melting temperatures were used to target a single homozygous allele in the HUMTH01 locus. The effect on amplification efficiency for each primer pair was determined by adding different concentrations of various PCR inhibitors to the reaction mixture. The results show that a variety of inhibition mechanisms can occur during the PCR process, depending on the type of co-extracted inhibitor. These include Taq inhibition, DNA template binding, and effects on reaction efficiency. In addition, some inhibitors appear to affect the reaction in more than one manner. Overall, we find that amplicon size and melting temperature are important in some inhibition mechanisms and not in others, and the key issue in understanding PCR inhibition is determining the identity of the interfering substance. 4 tables, 8 figures, and 21 references (Published abstract)
Main Term(s): Criminology
Index Term(s): DNA fingerprinting; Forensic sciences; Investigative techniques; Suspect identification; Victim identification
To cite this abstract, use the following link:
http://www.ncjrs.gov/App/publications/abstract.aspx?ID=251710

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