skip navigation

PUBLICATIONS

Register for Latest Research

Stay Informed
Register with NCJRS to receive NCJRS's biweekly e-newsletter JUSTINFO and additional periodic emails from NCJRS and the NCJRS federal sponsors that highlight the latest research published or sponsored by the Office of Justice Programs.

NCJRS Abstract

The document referenced below is part of the NCJRS Virtual Library collection. To conduct further searches of the collection, visit the Virtual Library. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.

 

NCJ Number: 229902 Find in a Library
Title: Forensic Method for the Simultaneous Analysis of Biallelic Markers Identifying Y Chromosome Haplogroups Inferred as Having Originated in Asia and the Japanese Archipelago
Journal: Forensic Science International: Genetics  Volume:4  Issue:2  Dated:February 2010  Pages:73-79
Author(s): Natsuko Mizuno; Tetsushi Kitayama; Koji Fujii; Hiroaki Nakahara; Kanako Yoshida; Kazumasa Sekiguchi; Naoto Yonezawa; Minoru Nakano; Kentaro Kasai
Date Published: February 2010
Page Count: 7
Publisher: http://www.elsevier.com 
Type: Report (Study/Research)
Format: Article
Language: English
Country: Ireland
Annotation: This article examines the ancestral and geographical origins of biological evidence samples.
Abstract: Information regarding the ancestral and geographical origins of biological evidence samples may be useful for crime investigators as they narrow down the possible donors of the sample. A method for simultaneous analysis of seven biallelic markers (M130, M131, M57, M125, M175, M122, and M134) was developed for forensic application. M57, M125, and M131 are included to identify haplogroups inferred as having originated in the Japanese archipelago. Our method employs allele-specific PCR and fragment analysis using fluorescently labeled primers and capillary electrophoresis. This method can be used to assign a haplogroup from both of degraded male DNA samples and DNA samples containing a mixture of female and male DNA by designing PCR primers that generate small amplicons and are highly specific for targets on the Y chromosome. A total of 1,346 samples from Japanese males collected from the four major islands and Okinawa island were classified into seven Y binary haplogroups, i.e., C-M130, C-M131, D-M57, D-M125, O-M175, O-M122, and O-M134, and a "no-mutation detected" group; and their frequencies were 0.0617, 0.0565, 0.1441, 0.182, 0.3418, 0.11, 0.0847, and 0.0193, respectively. Samples of "no-mutation detected" were further analyzed by direct sequencing for identification of the major haplogroup to which they belong. Along with the haplogroup data, we report haplotype data for the 16 Y-STR markers included in the AmpFISTR Yfiler TM PCR amplification kit (Applied Biosystems). These data will be useful in the prediction of haplogroups based on Y-STR haplotypes. Tables, figures, and references (Published Abstract)
Main Term(s): Criminology
Index Term(s): Asia; DNA fingerprinting; Foreign criminal justice research; Forensic sciences; Investigative techniques; Japan; Suspect identification; Victim identification
To cite this abstract, use the following link:
http://www.ncjrs.gov/App/publications/abstract.aspx?ID=251934

*A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's website is provided. Tell us how you use the NCJRS Library and Abstracts Database - send us your feedback.