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NCJ Number: 235780 Find in a Library
Title: Simplified Field Preservation of Tissues for Subsequent DNA Analyses
Journal: Journal of Forensic Sciences  Volume:56  Issue:4  Dated:July 2011  Pages:846-852
Author(s): Corinne L. Michaud, M.S.; David R. Foran, Ph.D.
Date Published: July 2011
Page Count: 7
Type: Report (Study/Research) ; Test/Measurement
Format: Article
Language: English
Country: United States of America
Annotation: Successful DNA-based identification of mass disaster victims depends on acquiring tissues that are not highly degraded. In this study, multiple protocols for field preservation of tissues for later DNA analysis were tested.
Abstract: Skin and muscle samples were collected from decaying pig carcasses. Tissues were preserved using cold storage, desiccation, or room temperature storage in preservative solutions for up to 6 months. DNA quality was assessed through amplification of successively larger segments of nuclear DNA. Solution-based storage, including a DMSO/NaCl/EDTA mixture, alcohols, and RNAlater preserved DNA of the highest quality, refrigeration was intermediate, and desiccation was least effective. Tissue type and extent of decomposition significantly affected stored DNA quality. Overall, the results indicate that any tissue preservation attempt is far superior to delaying or forgoing preservation efforts, and that simple, inexpensive methods can be highly effective in preserving DNA, thus should be initiated as quickly as possible. (Published Abstract)
Main Term(s): Criminology
Index Term(s): Disaster procedures; DNA fingerprinting; Evidence preservation; Forensic sciences; Investigative techniques; Victim identification
Note: Presented at the 58th Annual Meeting of the American Academy of Forensic Sciences, February 20-25, 2006, in Seattle, WA.
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