skip navigation

PUBLICATIONS

Register for Latest Research

Stay Informed
Register with NCJRS to receive NCJRS's biweekly e-newsletter JUSTINFO and additional periodic emails from NCJRS and the NCJRS federal sponsors that highlight the latest research published or sponsored by the Office of Justice Programs.

NCJRS Abstract

The document referenced below is part of the NCJRS Virtual Library collection. To conduct further searches of the collection, visit the Virtual Library. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.

 

NCJ Number: 235780 Find in a Library
Title: Simplified Field Preservation of Tissues for Subsequent DNA Analyses
Journal: Journal of Forensic Sciences  Volume:56  Issue:4  Dated:July 2011  Pages:846-852
Author(s): Corinne L. Michaud, M.S.; David R. Foran, Ph.D.
Date Published: July 2011
Page Count: 7
Publisher: http://www.wiley.com 
Type: Report (Study/Research) ; Test/Measurement
Format: Article
Language: English
Country: United States of America
Annotation: Successful DNA-based identification of mass disaster victims depends on acquiring tissues that are not highly degraded. In this study, multiple protocols for field preservation of tissues for later DNA analysis were tested.
Abstract: Skin and muscle samples were collected from decaying pig carcasses. Tissues were preserved using cold storage, desiccation, or room temperature storage in preservative solutions for up to 6 months. DNA quality was assessed through amplification of successively larger segments of nuclear DNA. Solution-based storage, including a DMSO/NaCl/EDTA mixture, alcohols, and RNAlater preserved DNA of the highest quality, refrigeration was intermediate, and desiccation was least effective. Tissue type and extent of decomposition significantly affected stored DNA quality. Overall, the results indicate that any tissue preservation attempt is far superior to delaying or forgoing preservation efforts, and that simple, inexpensive methods can be highly effective in preserving DNA, thus should be initiated as quickly as possible. (Published Abstract)
Main Term(s): Criminology
Index Term(s): Disaster procedures; DNA fingerprinting; Evidence preservation; Forensic sciences; Investigative techniques; Victim identification
Note: Presented at the 58th Annual Meeting of the American Academy of Forensic Sciences, February 20-25, 2006, in Seattle, WA.
To cite this abstract, use the following link:
http://www.ncjrs.gov/App/publications/abstract.aspx?ID=257767

*A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's website is provided. Tell us how you use the NCJRS Library and Abstracts Database - send us your feedback.