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NCJ Number: 236666 Find in a Library
Title: Evaluation of Novel Forensic DNA Storage Methodologies
Journal: Forensic Science International: Genetics  Volume:5  Issue:5  Dated:November 2011  Pages:386-392
Author(s): Christophe Frippiat; Sabrina Zorbo; Daniel Leonard; Anne Marcotte; Mariella Chaput; Charlotte Aelbrecht; Fabrice Noel
Date Published: November 2011
Page Count: 7
Type: Program/Project Evaluation
Format: Article
Language: English
Country: Ireland
Annotation: This study focused on the recent Gentegra product and initiated a comparison versus -20°C and Qiasafe storage.
Abstract: An issue in forensic sciences is the secure storage of extracted DNA. Most of the time, DNA is frozen at -20 °C or -80 °C. Recently, new room temperature DNA storage technologies have been developed based on anhydrobiosis. Two products use this technology: Qiasafe (Qiagen) and Gentegra (Genvault). In this study we focused on the recent Gentegra product and initiated a comparison versus -20 °C and Qiasafe storage. We compared the quantity and quality of DNA stored using anhydrobiosis technology against DNA stored at -20 °C, by performing STR profiling after short term storage. Furthermore, we studied the quantity and integrity of DNA after long term storage. Our results prove the high potential of this technology but it seems to be extraction dependent. Phenol/chloroform extracted DNA could be stored using the Gentegra matrix for more than 6 months without any obvious degradation. However, DNA extracted using magnetic beads could not be safely stored over the same period. Adaptations are therefore required to store this kind of samples.(Published Abstract)
Main Term(s): Forensic sciences
Index Term(s): Belgium; DNA fingerprinting; Evidence preservation; Foreign criminal justice research; Suspect identification; Victim identification
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