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NCJ Number: 236671 Find in a Library
Title: Development and Characterization of Two Mini-X Chromosomal Short Tandem Repeat Multiplexes
Journal: Forensic Science International: Genetics  Volume:5  Issue:5  Dated:November 2011  Pages:415-421
Author(s): Toni M. Diegoli; Michael D. Coble
Date Published: November 2011
Page Count: 7
Type: Report (Study/Research)
Format: Article
Language: English
Country: Ireland
Annotation: This study presented the development and characterization of two X chromosomal short tandem repeat (STR) multiplexes for identity and kinship testing with degraded DNA.
Abstract: This study presents the development and characterization of two X chromosomal short tandem repeat (STR) multiplexes utilizing reduced-size amplicons (less than 200 base pairs) for identity and kinship testing with degraded DNA. Aproximately 1,360 samples across 4 U.S. population groups were typed for 15 X chromosomal STR markers: DXS6789, DXS7130, DXS9902, GATA31E08, DXS7424, GATA165B12, DXS101, DXS6795, GATA172D05, DXS10147, DXS8378, DXS7132, DXS6803, HPRTB, and DXS7423. A high degree of polymorphism was observed for each marker and both multiplexes were sensitive down to 200pg of pristine DNA. The two proposed multiplexes are suitable for forensic use, and show potential for improved analysis of compromised bone samples. (Published Abstract)
Main Term(s): Forensic sciences
Index Term(s): DNA fingerprinting; Parentage determination; Suspect identification; United States of America; Victim identification
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