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NCJ Number: 236675 Find in a Library
Title: Selection of Highly Specific and Sensitive mRNA Biomarkers for the Identification of Blood
Journal: Forensic Science International: Genetics  Volume:5  Issue:5  Dated:November 2011  Pages:449-458
Author(s): C. Haas; E. Hanson; A. Kratzer; W. Bar; J. Ballantyne
Date Published: November 2011
Page Count: 10
Publisher: http://www.elsevier.com 
Type: Report (Study/Research)
Format: Article
Language: English
Country: Ireland
Annotation: This study evaluated eight reportedly blood-specific mRNA markers (HBB, HBA, ALAS2, CD3G, ANK1, PBGD, SPTB, AQP9) in an attempt to determine the most suitable ones for use in forensic applications based on their sensitivities, specificities and performance with casework samples.
Abstract: While varying levels of expression were observed, all markers were relatively sensitive requiring as little as 1ng of RNA input into the reverse transcription (RT) reaction. In singleplex reactions, seven of the eight analyzed blood markers (all except AQP9) demonstrated a high degree of specificity for blood. In multiplex reactions, non-reproducible cross-reactivity was observed for several of the mRNA markers, which was reduced and, in most cases, eliminated when less input total RNA was used. Additionally, some cross-reactivity was observed with tissue and animal samples. Despite differences in the observed sensitivity and specificity of the blood markers examined in this study, a number of the candidates appear to be suitable for inclusion in appropriately validated multiplex mRNA-based body fluid identification systems. (Published Abstract)
Main Term(s): Forensic sciences
Index Term(s): Blood stains; Blood/body fluid analysis; DNA fingerprinting; Investigative techniques; Suspect identification; United States of America; Victim identification
To cite this abstract, use the following link:
http://www.ncjrs.gov/App/publications/abstract.aspx?ID=258690

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