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NCJ Number: 237517 Find in a Library
Title: Transfer of Touch DNA From Hands to Glass, Fabric and Wood
Journal: Forensic Science International: Genetics  Volume:6  Issue:1  Dated:January 2012  Pages:41-46
Author(s): Dyan J. Daly; Charlotte Murphy; Sean D. McDermott
Date Published: January 2012
Page Count: 6
Type: Report (Study/Research)
Format: Article
Language: English
Country: Ireland
Annotation: The transfer of DNA from hands to objects by holding or touching has been examined in the past. The main purpose of this study was to examine the variation in the amount of DNA transferred from hands to glass, fabric and wood.
Abstract: The study involved 300 volunteers (100 for glass, 100 for fabric and 100 for wood) 50 percent of which were male and 50 percent female. The volunteers held the material for 60s. The DNA was recovered from the objects using a minitape lift, quantified using the Quantifiler kit assay, extracted using a 'Qiagen(®) QIAamp DNA mini kit' and amplified using the AmpFlSTR(®) SGM Plus™ Amplification Kit at 28 cycles. The results show that using ANOVA there was a significant difference (F=8.2, p less than 0.05) between the three object types in the amount of DNA recovered. In terms of DNA transfer and recovery, wood gave the best yield, followed by fabric and then glass. The likelihood of success of obtaining a profile indicative of the holder was approximately 9 percent for glass samples, 23 percent for fabric and 36 percent for wood. There was no significant difference between the amount of DNA transferred by male or female volunteers. In this study good shedder status, as defined by obtaining useful profiles of six or more alleles, is estimated at approximately 22 percent of the population. The phenomenon of secondary transfer was observed when mixed DNA profiles were obtained but the incidence was low at approximately 10 percent of the total number of samples. DNA profiles corresponding to more than one person were found on objects which had been touched by only one volunteer. Although secondary transfer is possible the profiles obtained from touched objects are more likely to be as a result of primary transfer rather than a secondary source. (Published Abstract)
Main Term(s): Police policies and procedures
Index Term(s): Blood/body fluid analysis; DNA fingerprinting; Fingerprints; Foreign criminal justice research; Forensic sciences; Investigative techniques; Latent fingerprints; Suspect identification
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