skip navigation

PUBLICATIONS

Register for Latest Research

Stay Informed
Register with NCJRS to receive NCJRS's biweekly e-newsletter JUSTINFO and additional periodic emails from NCJRS and the NCJRS federal sponsors that highlight the latest research published or sponsored by the Office of Justice Programs.

NCJRS Abstract

The document referenced below is part of the NCJRS Virtual Library collection. To conduct further searches of the collection, visit the Virtual Library. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.

 

NCJ Number: 237523 Find in a Library
Title: Development of a One-Tube Extraction and Amplification Method for DNA Analysis of Sperm and Epithelial Cells Recovered From Forensic Samples by Laser Microdissection
Journal: Forensic Science International: Genetics  Volume:6  Issue:1  Dated:January 2012  Pages:91-96
Author(s): Melanie Meredith; Jo-Anne Bright; Sarah Cockerton; Sue Vintiner
Date Published: January 2012
Page Count: 6
Publisher: http://www.elsevier.com 
Type: Report (Study/Research)
Format: Article
Language: English
Country: Ireland
Annotation: In this paper, the development of a one-tube method for the effective extraction of DNA from laser microdissected sperm and epithelial cells is described.
Abstract: Laser microdissection can be used in forensic casework to isolate specific cell types from mixtures of biological samples. Extraction of DNA from selected cells is still required prior to STR amplification. Because of the relatively pristine nature of the recovered cells, laser microdissection is more sensitive than more traditional methods of DNA analysis, theoretically resulting in DNA profiles from less cellular material. A one-tube extraction and amplification method minimises loss of DNA through liquid transfers and reduces the potential for contamination events occurring. In this paper, the development of a one-tube method for the effective extraction of DNA from laser microdissected sperm and epithelial cells is described. The performance of the in-house method was compared to that of a commercial DNA extraction kit for extraction of DNA from sperm and the downstream compatibility with STR amplification was determined for both sperm and epithelial samples. Full Identifiler™ profiles after 28 amplification cycles were obtained from as few as 15 epithelial cells and 30 sperm. (Published Abstract)
Main Term(s): Police policies and procedures
Index Term(s): Blood/body fluid analysis; DNA fingerprinting; Foreign criminal justice research; Forensic sciences; Investigative techniques; New Zealand; Suspect identification
To cite this abstract, use the following link:
http://www.ncjrs.gov/App/publications/abstract.aspx?ID=259554

*A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's website is provided. Tell us how you use the NCJRS Library and Abstracts Database - send us your feedback.